Institute of Clinical Biochemistry and Diagnostics, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Department of Neurology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czech Republic.
Eur J Pharmacol. 2018 Apr 5;824:115-119. doi: 10.1016/j.ejphar.2018.02.010. Epub 2018 Feb 8.
Some data suggest that exposure to levetiracetam (LEV) might be associated with a risk for bone health in the model of orchidectomized rats. The aim of this study was to investigate if there is any significant risk of LEV for bone health in the model of gonadally intact animals. Wistar rats were divided into a control group and a test group, 8 rats in each group. The control rats received standard laboratory diet (SLD) while rats in the test group were fed SLD enriched with LEV for 12 weeks. Dual energy X-ray absorptiometry was used to measure BMD of the whole body, femur and lumbar vertebrae. The concentrations of bone markers were examined in bone homogenate. Both femurs and tibiae were used for biomechanical testing. We found in the LEV group significantly decreased absolute and relative values of adipose tissue, higher whole-body BMD, higher right tibia cortical thickness, and a significantly increased concentration of Bone Alkaline Phosphatase (BALP) and cross-linked C-telopeptide of type I collagen (CTX-I) compared with the control group. The results suggest that the long-term administration of LEV in the model of gonadally intact rats does not have a negative effect on bone. Significant increase in BMD and cortical thickness of the right tibia may indicate even a positive influence on the properties of bone. Further studies will be necessary in animals and humans to confirm these findings.
一些数据表明，左乙拉西坦（LEV）的暴露可能与去势大鼠模型中的骨健康风险有关。本研究旨在探讨在性腺完整动物模型中，LEV 是否对骨健康存在显著风险。Wistar 大鼠分为对照组和实验组，每组 8 只。对照组大鼠给予标准实验室饮食（SLD），实验组大鼠给予 SLD 加 LEV 喂养 12 周。双能 X 线吸收法用于测量全身、股骨和腰椎的 BMD。骨匀浆中检查骨标志物的浓度。对双侧股骨和胫骨进行生物力学测试。我们发现，LEV 组的脂肪组织绝对值和相对值明显降低，全身 BMD 升高，右侧胫骨皮质厚度增加，骨碱性磷酸酶（BALP）和 I 型胶原交联 C 端肽（CTX-I）的浓度明显升高，与对照组相比。结果表明，在性腺完整大鼠模型中，长期给予 LEV 对骨骼没有负面影响。右侧胫骨 BMD 和皮质厚度的显著增加可能表明对骨骼的特性甚至有积极的影响。为了证实这些发现，还需要在动物和人类中进行进一步的研究。
