College of Environmental Science and Engineering, Hunan University, Changsha 410082, China.
Department of Urban Water Environmental Research, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Bioresour Technol. 2018 May;256:69-76. doi: 10.1016/j.biortech.2018.01.146. Epub 2018 Feb 2.
A lab-scale two-stage Anoxic/Oxic with MBR (AO/AO-MBR) system was operated for 81 days for leachate treatment with different reflux ratio (R). The best system performances were observed with a R value of 150%, and the average removal efficiencies of chemical oxygen demand, ammonia and total nitrogen were 85.6%, 99.1%, and 77.6%, respectively. The microbial community were monitored and evaluated using high-throughput sequencing. Proteobacteria were dominant in all process. Phylogenetic trees were described at species level, genus Thiopseudomonas, Amaricoccus, Nitrosomonas and Nitrobacter played significant roles in nitrogen removal. Co-occurrence analyzing top 20 genera showed that Nitrosomonas-Nitrobacter presented perfect positive relationship, as well as Paracoccus-Brevundimonas and Pusillimonas-Halobacteriovorax.
采用实验室规模两级缺氧/好氧与膜生物反应器(AO/AO-MBR)系统,以不同回流比(R)运行 81 天来处理渗滤液。当 R 值为 150%时,观察到最佳的系统性能,化学需氧量、氨和总氮的平均去除效率分别为 85.6%、99.1%和 77.6%。使用高通量测序监测和评估微生物群落。在所有过程中,变形菌门都是优势菌门。系统发育树在物种水平上进行了描述,硫假单胞菌属、阿玛瑞球菌属、亚硝化单胞菌属和硝化杆菌属在氮去除中发挥了重要作用。前 20 个属的共现分析表明,亚硝化单胞菌属-硝化杆菌属呈完美的正相关关系,副球菌属-短小杆菌属和普西利蒙斯菌属-盐杆菌属也是如此。