School of Civil Engineering, Wuhan University, Wuhan 430072, China; School of Civil Engineering, Nanyang Institute of Technology, Nanyang 473004, China.
School of Civil Engineering, Wuhan University, Wuhan 430072, China.
Bioresour Technol. 2020 Jan;296:122344. doi: 10.1016/j.biortech.2019.122344. Epub 2019 Oct 28.
In this work, a pilot-scale low dissolved oxygen (DO) composite biological system (LDOCBS) composed of an anoxic rotating biological contactor (RBC) and four aeration tanks with gradient aeration was used to treat landfill leachate for 88 d. The maximum removals of 85.65%, 99.92% and 84.06% for chemical oxygen demand (COD), ammonia (NH-N) and total nitrogen (TN) were achieved, respectively. The three-dimensional exaction and emission matrix (3D-EEM) fluorescence spectroscopy revealed that the biodegradability of leachate was significantly improved by the LDOCBS. Mass balance calculations showed that the COD removal and denitrification process mainly occurred in RBC while 1# contributed primarily to nitrification. High-throughput sequencing analysis indicated that denitrifying bacteria with highly relative abundances of 46.45%-53.81% played key roles in organic degradation and nitrogen removal. This work could add some guiding insights into the cost-efficient treatment of landfill leachate by the composite biological system.
在这项工作中，采用了由缺氧旋转生物接触器（RBC）和四个带有梯度曝气的曝气池组成的中试规模低溶解氧（DO）复合生物系统（LDOCBS），对垃圾渗滤液进行了 88 天的处理。COD、氨氮（NH-N）和总氮（TN）的最大去除率分别达到了 85.65%、99.92%和 84.06%。三维萃取和发射矩阵（3D-EEM）荧光光谱表明，LDOCBS 显著提高了渗滤液的可生物降解性。质量平衡计算表明，COD 的去除和反硝化过程主要发生在 RBC 中，而 1# 主要负责硝化作用。高通量测序分析表明，具有相对丰度为 46.45%-53.81%的反硝化菌在有机降解和脱氮过程中发挥了关键作用。这项工作可以为复合生物系统低成本处理垃圾渗滤液提供一些指导。
