Heart Center, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong 510006, PR China.
Department of Critical-Care Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, Guangdong 510006, PR China.
Biomed Pharmacother. 2018 Apr;100:156-167. doi: 10.1016/j.biopha.2018.02.013. Epub 2018 Feb 8.
The study aimed to investigate whether a Shengmai San-derived herbal, Fumai granule (FM), which had a preventive effect on atrial fibrillation (AF) in myocardial infarction (MI)-induced heart failure (HF) rat and to determine the underlying mechanisms.
MI was induced by a ligation of the left anterior descending coronary artery. One week after MI surgery, FM was gavaged for 4 weeks. AF inducibility was detected by transesophageal programmed electrical stimulation technology. Multielectrode array measurements, echocardiogram, histology, and western blotting were performed.
The FM-treated group had lower rates of AF inducibility and shorter AF duration compared to the MI group. FM improved the conduction velocity and homogeneity, decreased left atrial positive fibrosis areas and expression of type I and III collagen, inhibited cardiac fibroblast to myofibroblast differential, and increased the expression of connexin 43 and connexin 40 in the left atrium.
These results suggest that FM reduced the AF inducibility after MI by improving the left atrial conduction function via inhibiting left atrial fibrosis and increasing the expression of connexin, indicating its benefit in preventing the MI-induced vulnerable substrate for AF.
本研究旨在探讨生脉散衍生草药复脉颗粒（FM）是否对心肌梗死后心力衰竭（HF）大鼠的心房颤动（AF）具有预防作用，并探讨其潜在机制。
通过结扎左前降支冠状动脉诱导 MI。MI 手术后 1 周，给予 FM 灌胃 4 周。通过经食管程控电刺激技术检测 AF 易感性。进行多电极阵列测量、超声心动图、组织学和 Western blot 分析。
与 MI 组相比，FM 处理组的 AF 易感性发生率较低，AF 持续时间较短。FM 改善了传导速度和均匀性，减少了左心房阳性纤维化面积和 I 型和 III 型胶原的表达，抑制了心肌成纤维细胞向肌成纤维细胞的分化，并增加了左心房中连接蛋白 43 和连接蛋白 40 的表达。
这些结果表明，FM 通过抑制左心房纤维化和增加连接蛋白的表达来改善左心房传导功能，从而降低 MI 后 AF 的易感性，表明其在预防 MI 诱导的 AF 易损基质方面具有益处。
