Wang Jie, Gong Xiaowen, Tian Geng G, Hou Changliang, Zhu Xiaoqin, Pei Xiuying, Wang Yanrong, Wu Ji
Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Ningxia Medical University, Yinchuan 750004, China.
Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai 200240, China.
Gene. 2018 May 5;653:14-21. doi: 10.1016/j.gene.2018.02.021. Epub 2018 Feb 8.
Female germline stem cells (FGSCs) are proposed to be a key factor for ameliorating female infertility. Previously we have shown that neonatal and adult FGSCs could be isolated and purified from mouse ovarian tissues. The long noncoding (lnc) RNA growth arrest-specific 5 sequence (GAS5) transcribed from mammalian genomes plays important regulatory roles in various developmental processes. However, there is no study on the relationship between GAS5 and FGSC development in vitro. In this study, we showed that GAS5 was highly expressed in the neonatal mouse ovary and was located in both FGSCs and oocytes. GAS5 facilitated FGSC proliferation and promoted their survival in vitro. Moreover, GAS5 also inhibited apoptosis of cultured FGSCs. These findings indicate that GAS5 is a crucial regulator of FGSC development. This might serve as a foundation for a strategy of lncRNA-directed diagnosis or treatment of female infertility.
雌性生殖系干细胞（FGSCs）被认为是改善女性不孕症的关键因素。此前我们已表明，可从小鼠卵巢组织中分离并纯化出新生和成年FGSCs。从哺乳动物基因组转录而来的长链非编码（lnc）RNA生长停滞特异性5序列（GAS5）在各种发育过程中发挥着重要的调控作用。然而，关于GAS5与体外FGSC发育之间的关系尚无研究。在本研究中，我们发现GAS5在新生小鼠卵巢中高表达，且位于FGSCs和卵母细胞中。GAS5促进FGSC增殖并在体外促进其存活。此外，GAS5还抑制培养的FGSCs凋亡。这些发现表明GAS5是FGSC发育的关键调节因子。这可能为lncRNA导向的女性不孕症诊断或治疗策略奠定基础。
