Walukiewicz Hanna E, Ordal George W, Rao Christopher V
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Department of Medical Biochemistry, University of Illinois, Urbana, IL, USA.
Methods Mol Biol. 2018;1729:95-105. doi: 10.1007/978-1-4939-7577-8_10.
The sensing apparatus of the Bacillus subtilis chemotaxis pathway involves a complex consisting of chemoreceptors, the CheA histidine kinase, and the CheV and CheW adaptor proteins. Attractants and repellents alter the rate of CheA autophosphorylation, either by directly binding the receptors or by indirectly interacting with them through intermediate binding proteins. We describe an in vitro assay for measuring receptor-kinase activity in B. subtilis. This assay has been used to investigate the mechanism of signal transduction in B. subtilis chemotaxis and the disparate mechanisms employed by this bacterium for sensory adaptation and gradient sensing.
枯草芽孢杆菌趋化途径的传感装置涉及一个由化学感受器、CheA组氨酸激酶以及CheV和CheW衔接蛋白组成的复合体。引诱剂和驱避剂通过直接结合受体或通过中间结合蛋白与受体间接相互作用，来改变CheA自身磷酸化的速率。我们描述了一种用于测量枯草芽孢杆菌中受体激酶活性的体外检测方法。该检测方法已被用于研究枯草芽孢杆菌趋化作用中的信号转导机制，以及该细菌用于感觉适应和梯度感知的不同机制。
