Chen T, Zhang W, Liang Y, Li Q, Yang C, Yuan Y X, Ban M L
Department of Otorhinolaryngology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Feb 7;53(2):118-123. doi: 10.3760/cma.j.issn.1673-0860.2018.02.007.
To investigate the effect of melatonin on the expression of prestin protein in the inner ear of mice following a single dose radiation therapy, so as to provide the basis for the mechanism study of radiation induced inner ear injury and its prevention. Sixty 4-week-old male mice were randomly divided into six groups, including the control group (A group), 50 mg/kg MLT group (B group), 5 mg/kg MLT group (C group), 50 mg/kg MLT + radiotherapy group (D group), 5 mg/kg MLT+ radiotherapy group (E group), and 16 Gy radiotherapy group (F group). Each experimental group was randomly subdivided into two subgroups, which were killed to harvest the cochlea on the 3rd and 7th days following 16 Gy radiation. The specimens were used for immunostaining and Western blot to detect the expression of prestin protein. SPSS 19.0 software was used for statistical analysis. Prestin protein mainly distributed in the lateral membrane above the outer hair cell nucleus. When compared with A, B and C group, the expression of prestin protein in the inner ear was significantly up-regulated in F group (<0.05). However, D and E group reduced the abnormal expression of prestin following radiotherapy when compared with F group, the difference was statistically significant (<0.05), and the effect of D group was more significant than E group (<0.05). The prestin protein of cochlea is mainly distributed in the lateral membrane above the outer hair cell nucleus. Following the high-dose radiotherapy, the prestin expression is upregulated, and melatonin can control the abnormal expression of prestin protein induced by radiotherapy with dose dependent.
探讨褪黑素对单次剂量放射治疗后小鼠内耳中prestin蛋白表达的影响，为辐射性内耳损伤的机制研究及其预防提供依据。将60只4周龄雄性小鼠随机分为6组，包括对照组（A组）、50mg/kg褪黑素组（B组）、5mg/kg褪黑素组（C组）、50mg/kg褪黑素+放射治疗组（D组）、5mg/kg褪黑素+放射治疗组（E组）和16Gy放射治疗组（F组）。每个实验组再随机分为两个亚组，于16Gy辐射后第3天和第7天处死小鼠以获取耳蜗。标本用于免疫染色和蛋白质印迹法检测prestin蛋白的表达。采用SPSS 19.0软件进行统计分析。Prestin蛋白主要分布在外毛细胞核上方的侧膜上。与A、B、C组相比，F组内耳中prestin蛋白表达显著上调（<0.05）。然而，与F组相比，D组和E组放疗后prestin的异常表达降低，差异有统计学意义（<0.05），且D组效果比E组更显著（<0.05）。耳蜗的prestin蛋白主要分布在外毛细胞核上方的侧膜上。高剂量放疗后，prestin表达上调，褪黑素可剂量依赖性地控制放疗诱导的prestin蛋白异常表达。
