Fleming Damarius S, Miller Laura C
ORAU/ORISE, Oak Ridge, TN, USA; Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, P.O. Box 70, Ames, IA 50010-0070, USA.
Virus and Prion Diseases of Livestock Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, P.O. Box 70, Ames, IA 50010-0070, USA.
Virology. 2018 Apr;517:56-61. doi: 10.1016/j.virol.2018.01.027. Epub 2018 Feb 21.
It has been established that reduced susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV) has a genetic component. This genetic component may take the form of small non-coding RNAs (sncRNA), which are molecules that function as regulators of gene expression. Various sncRNAs have emerged as having an important role in the immune system in humans. The study uses transcriptomic read counts to profile the type and quantity of both well and lesser characterized sncRNAs, such as microRNAs and small nucleolar RNAs to identify and quantify the classes of sncRNA expressed in whole blood between healthy and highly pathogenic PRRSV-infected pigs. Our results returned evidence on nine classes of sncRNA, four of which were consistently statistically significantly different based on Fisher's Exact Test, that can be detected and possibly interrogated for their effect on host dysregulation during PRRSV infections.
已经确定，对猪繁殖与呼吸综合征病毒（PRRSV）的易感性降低具有遗传成分。这种遗传成分可能以小非编码RNA（sncRNA）的形式存在，sncRNA是作为基因表达调节因子发挥作用的分子。各种sncRNA已被证明在人类免疫系统中具有重要作用。该研究使用转录组读数来分析特征明确和特征不太明确的sncRNA的类型和数量，如微小RNA和小核仁RNA，以识别和量化健康猪和高致病性PRRSV感染猪全血中表达的sncRNA类别。我们的结果提供了关于九类sncRNA的证据，其中四类基于Fisher精确检验在统计学上始终存在显著差异，这些sncRNA在PRRSV感染期间对宿主失调的影响可以被检测到并可能进行研究。
