College of Food Science and Technology and MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
College of Life Sciences and Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang 438000, China.
Ultrason Sonochem. 2018 Apr;42:823-831. doi: 10.1016/j.ultsonch.2017.12.022. Epub 2017 Dec 14.
The differences between ultrasonic and non-ultrasonic approaches in synthesizing Lignosus rhinocerotis polysaccharide-selenium nanoparticles (LRP-SeNPs) were compared in terms of size, morphology, stability and antioxidant activity by UV-VIS, FT-IR, X-ray diffraction (XRD), dynamic light scattering (DLS), transmission electron microscopy (TEM), and energy dispersive X-ray (EDX) with high resolution TEM. Results indicated that the SeNPs were associated with the LRP macromolecules in a physical adsorption pattern without breaking chemical bonds, and the ultrasonic treatment reduced the size of SeNPs, narrowed the size distribution as well as improved the stability. Due to the LRP compact coil structure loosed under ultrasonic cavitation, the SeNPs could be easily diffused into the LRP internal branches instead of gathering on the LRP surface and were well dispersed and eventually stabilized throughout the extended branches. After ultrasound treatment, the SeNPs had a minimum average diameter of ∼50 nm and the LRP-SeNPs could remain homogeneous and translucent for 16 days within 200 nm size. Furthermore, the ultrasound-treated LRP-SeNPs exhibited higher DPPH and ABTS radical-scavenging abilities than those untreated with ultrasound. This difference may be attributed to the reason that ultrasound can reduce the SeNPs size and increase the specific surface area, which provides sufficient active sites to react with the free radicals and suppress the oxidizing reactions. The integrated results demonstrated that ultrasound played a crucial role in the dispersion, size control, stabilization and antioxidant activity of SeNPs.
超声和非超声方法在合成灵芝多糖-硒纳米粒子（LRP-SeNPs）方面的差异，通过紫外可见分光光度计（UV-VIS）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、动态光散射（DLS）、透射电子显微镜（TEM）和高分辨率透射电子显微镜（HR-TEM）进行了比较，考察了粒径、形态、稳定性和抗氧化活性。结果表明，硒纳米粒子与 LRP 大分子以物理吸附模式结合，而不破坏化学键，超声处理减小了 SeNPs 的粒径，缩小了粒径分布，提高了稳定性。由于 LRP 紧密的螺旋结构在超声空化下松弛，SeNPs 可以很容易地扩散到 LRP 的内部分支中，而不是聚集在 LRP 表面，并在扩展的分支中均匀分散和稳定。超声处理后，SeNPs 的平均直径最小可达∼50nm，在 200nm 尺寸范围内，LRP-SeNPs 可保持均匀透明状态达 16 天。此外，经超声处理的 LRP-SeNPs 对 DPPH 和 ABTS 自由基的清除能力高于未经超声处理的 LRP-SeNPs。这种差异可能是由于超声可以减小 SeNPs 的粒径，增加比表面积，为与自由基反应提供足够的活性位点，抑制氧化反应。综合结果表明，超声在 SeNPs 的分散、粒径控制、稳定和抗氧化活性方面起着关键作用。
