Bhiri Nour, Masquelez Nathalie, Nasri Moncef, Nasri Rim, Hajji Mohamed, Li Suming
Institut Européen des Membranes, IEM, UMR 5635, University of Montpellier, CNRS, ENSCM, 34095 Montpellier, France.
Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P.O. Box 1173, Sfax 3038, Tunisia.
Nanomaterials (Basel). 2025 Mar 12;15(6):435. doi: 10.3390/nano15060435.
Selenium nanoparticles (SeNPs) attract considerable attention for their promising applications in the biomedical field, driven by their unique properties and antioxidant activities. However, their practical use is often hindered by issues such as instability and aggregation. In this study, a polysaccharide, P2, extracted from , was used to stabilize SeNPs to address these limitations. P2-SeNPs were prepared through a green synthesis method involving sodium selenite, P2, and ascorbic acid, and characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier-transform infrared (FT-IR) spectroscopy, and X-ray diffraction (XRD). P2-SeNPs exhibited a smaller particle size and enhanced stability compared to unmodified SeNPs. UV-Vis spectroscopy and X-ray photoelectron spectroscopy (XPS) demonstrated the presence of Se-O bonds, suggesting effective stabilization by covalent bonding between SeNPs and P2. Stability tests revealed that P2-SeNPs maintained good dispersion under various conditions, with optimal stability observed at refrigerated temperatures and neutral pH. Moreover, P2-SeNPs exhibited better antioxidant activities than unmodified SeNPs, as evidenced by higher DPPH radical scavenging, ABTS radical scavenging, and metal chelation ratios. This difference is attributed to both the reduced aggregation and smaller size of P2-SeNPs. Therefore, it is concluded that P2-SeNPs exhibit significant potential as an effective antioxidant agent for biomedical applications.
硒纳米颗粒（SeNPs）因其独特的性质和抗氧化活性，在生物医学领域具有广阔的应用前景，因而备受关注。然而，其实际应用常常受到稳定性和聚集等问题的阻碍。在本研究中，从[具体来源未给出]提取的一种多糖P2被用于稳定SeNPs，以解决这些局限性。通过一种绿色合成方法，利用亚硒酸钠、P2和抗坏血酸制备了P2-SeNPs，并通过动态光散射（DLS）、透射电子显微镜（TEM）、傅里叶变换红外（FT-IR）光谱和X射线衍射（XRD）对其进行了表征。与未修饰的SeNPs相比，P2-SeNPs呈现出更小的粒径和更高的稳定性。紫外可见光谱和X射线光电子能谱（XPS）证明了Se-O键的存在，表明SeNPs与P2之间通过共价键实现了有效的稳定。稳定性测试表明，P2-SeNPs在各种条件下均保持良好的分散性，在冷藏温度和中性pH条件下观察到最佳稳定性。此外，P2-SeNPs表现出比未修饰的SeNPs更好的抗氧化活性，这通过更高的DPPH自由基清除率、ABTS自由基清除率和金属螯合率得以证明。这种差异归因于P2-SeNPs的聚集减少和粒径变小。因此，可以得出结论，P2-SeNPs作为一种有效的生物医学应用抗氧化剂具有巨大的潜力。
