盐皮质激素受体拮抗剂对大鼠失血性休克反应的影响。
Effects of mineralocorticoid receptor antagonists on responses to hemorrhagic shock in rats.
作者信息
Yamamoto Kanako, Yamamoto Takashi, Takamura Masayuki, Usui Soichiro, Murai Hisayoshi, Kaneko Shuichi, Taniguchi Takumi
机构信息
Department of System Biology, Kanazawa University Graduate School of Advanced Preventive Medical Sciences, Kanazawa University, Kanazawa 920-8641, Japan.
Department of Anesthesiology and Intensive Care Medicine, Kanazawa University Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8641, Japan.
出版信息
World J Crit Care Med. 2018 Feb 4;7(1):1-8. doi: 10.5492/wjccm.v7.i1.1.
AIM
To evaluate the effects of mineralocorticoid receptor (MR) antagonists on mortality and inflammatory responses after hemorrhagic shock (HS) in rats.
METHODS
One hundred and two male Sprague-Dawley rats were randomly assigned to one of the following three groups: Control, spironolactone (SPL), and eplerenone (EP) groups. HS was induced by the removal of blood. One half of rats were evaluated to determine mortality, hemodynamics, plasma tumor necrosis factor-alpha (TNF-α) concentrations, and arterial blood gas at 8 h after HS recovery. In the remainder of rats, the expression levels of genes encoding cytokines were evaluated in liver tissue samples at 1 h after HS recovery.
RESULTS
The survival rates 8 h after HS recovery were 71%, 94%, and 82% in the control, SPL, and EP groups, respectively. There were no significant differences in survival rates among the three groups ( = 0.219). Furthermore, there were no significant differences in gene expression levels in the liver or plasma TNF-α concentrations among the three groups ( = 0.888).
CONCLUSION
Pretreatment with MR antagonists did not improve mortality or cytokine responses in the liver after HS recovery in rats.
目的
评估盐皮质激素受体（MR）拮抗剂对大鼠失血性休克（HS）后死亡率和炎症反应的影响。
方法
102只雄性Sprague-Dawley大鼠被随机分为以下三组之一：对照组、螺内酯（SPL）组和依普利酮（EP）组。通过放血诱导HS。一半的大鼠在HS恢复后8小时进行评估，以确定死亡率、血流动力学、血浆肿瘤坏死因子-α（TNF-α）浓度和动脉血气。在其余大鼠中，在HS恢复后1小时评估肝组织样本中细胞因子编码基因的表达水平。
结果
HS恢复后8小时，对照组、SPL组和EP组的存活率分别为71%、94%和82%。三组之间的存活率无显著差异（=0.219）。此外，三组之间肝脏中的基因表达水平或血浆TNF-α浓度也无显著差异（=0.888）。
结论
MR拮抗剂预处理并未改善大鼠HS恢复后的死亡率或肝脏中的细胞因子反应。
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