Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, 226001, India.
Pesticide Toxicology Laboratory, Regulatory Toxicology and Nanomaterial Toxicology Group, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Vishvigyan Bhawan, 31 Mahatma Gandhi Marg, Lucknow, Uttar Pradesh, 226001, India.
Anal Bioanal Chem. 2018 Mar;410(8):2241-2251. doi: 10.1007/s00216-018-0894-0. Epub 2018 Feb 12.
Phenolic resin based activated carbon fibers (ACFs) were applied for the first time as a reversed-dispersive solid-phase extraction (r-DSPE) sorbent. A modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) method was applied to determine 26 pesticides (organophosphates, organochlorines, synthetic pyrethroids, and herbicides) in different complex matrices, including cauliflower, cucumber, banana, apple, wheat, and black gram. Different physicochemical characterization techniques were used to investigate the engineering and structural properties of the r-DSPE sorbent. All the chromatographic analyses were performed with a gas chromatograph equipped with an electron capture detector. The recoveries of all 26 pesticides were acceptable (70-120%), with relative standard deviations of less than 15%. The limit of detection and the limit of quantification were 1.13-5.48 ng/g and 3.42-16.60 ng/g, respectively. In the original QuEChERS method, primary secondary amine is extensively used as the r-DSPE sorbent in the cleanup process, but it is eightfold more expensive than the ACFs used in this study. Therefore, the modified QuEChERS method using ACFs during the cleanup process is more efficient, cheaper, and more robust to determine pesticides from different types of matrices, including vegetables, grains, and fruits, and ACFs could be used as a cost-effective alternative to primary secondary amine. Graphical Abstract Sample clean-up using PSA and ACF as r-DSPE sorbent in QuEChERS method.
酚醛树脂基活性炭纤维（ACF）首次被应用于反相分散固相萃取（r-DSPE）吸附剂。改良后的快速、简便、廉价、有效、耐用和安全（QuEChERS）方法被应用于测定不同复杂基质（包括花椰菜、黄瓜、香蕉、苹果、小麦和黑大豆）中的 26 种农药（有机磷、有机氯、合成拟除虫菊酯和除草剂）。采用不同的物理化学特性分析技术来研究 r-DSPE 吸附剂的工程和结构特性。所有的色谱分析都是在配备电子俘获检测器的气相色谱仪上进行的。所有 26 种农药的回收率均在可接受范围内（70-120%），相对标准偏差小于 15%。检出限和定量限分别为 1.13-5.48ng/g 和 3.42-16.60ng/g。在原始的 QuEChERS 方法中，主要使用伯仲胺作为 r-DSPE 吸附剂来进行净化过程，但它比本研究中使用的 ACF 昂贵八倍。因此，改良后的 QuEChERS 方法在净化过程中使用 ACF 来测定不同类型基质（包括蔬菜、谷物和水果）中的农药更有效、更经济、更耐用，ACF 可以作为伯仲胺的经济替代品。 摘要图 采用 PSA 和 ACF 作为 r-DSPE 吸附剂的 QuEChERS 法进行样品净化。
