Laboratório de Virologia Aplicada, Programa de Pós-Graduação em Biotecnologia e Biociências, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
Laboratório de Farmacognosia, Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.
J Sci Food Agric. 2018 Aug;98(11):4331-4338. doi: 10.1002/jsfa.8959. Epub 2018 Apr 18.
Phytochemical profiles of two Brazilian native fruits - pitanga (red and purple) and araçá (yellow and red) - as well as strawberry cultivars Albion, Aromas and Camarosa, blackberry cultivar Tupy and blueberry cultivar Bluegen cultivated in Brazil were characterized for total phenolic content and total anthocyanin content by liquid chromatography coupled to a photodiode array and a quadrupole time-of-flight mass spectrometer. Radical scavenging, antiherpes and cytotoxic activities of these berry extracts were also evaluated.
Blueberry presented the highest total anthocyanin content (1202 mg cyanidin-O-glucoside equivalents kg fresh fruit), while strawberry cultivar Aromas presented the highest total phenolic content (13 550 mg gallic acid equivalents kg fresh fruit). Liquid chromatographic-mass spectrometric analysis resulted in the identification of 21 anthocyanins. To the best of our knowledge this is the first report of cyanidin-O-glucoside in yellow and red Araçá fruit and the first time eight anthocyanins have been reported in pitanga fruits. DPPH and ABTS assays showed that blueberry cultivar Bluegen, blackberry cultivar Tupy and pitanga (red and purple) showed the most promising antiradical activities, respectively. No relevant cytotoxicity against three cancer cell lines or antiherpes activity was detected under the experimental conditions tested.
Total anthocyanin content of all fruits had a strong positive correlation with their free radical scavenging activity, suggesting anthocyanins contribute to the antioxidant potential of these fruits. © 2018 Society of Chemical Industry.
两种巴西本土水果——彭桃（红、紫）和阿萨伊（黄、红）以及草莓品种阿尔比恩、阿罗玛斯和卡梅罗萨、黑莓品种图皮和蓝莓品种布鲁根在巴西种植，其总酚含量和总花青素含量采用液相色谱-光电二极管阵列-四极杆飞行时间质谱联用进行了测定。还评估了这些浆果提取物的自由基清除、抗疱疹和细胞毒性活性。
蓝莓的总花青素含量最高（1202mg 矢车菊素-O-葡萄糖苷当量/kg 新鲜果实），而草莓品种阿罗玛斯的总酚含量最高（13550mg 没食子酸当量/kg 新鲜果实）。液质联用分析鉴定出 21 种花色苷。据我们所知，这是首次报道黄、红阿萨伊果中有矢车菊素-O-葡萄糖苷，也是首次在彭桃果中报道 8 种花色苷。DPPH 和 ABTS 测定表明，蓝莓品种布鲁根、黑莓品种图皮和彭桃（红、紫）具有最强的自由基清除活性。在实验测试条件下，未检测到对三种癌细胞系的细胞毒性或抗疱疹活性。
所有水果的总花青素含量与其自由基清除活性呈强正相关，表明花色苷有助于这些水果的抗氧化潜力。©2018 化学工业协会。
