Department for Biosystems Science and Engineering (D-BSSE), Eidgenössische Technische Hochschule (ETH) Zurich, 4058, Basel, Switzerland.
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Chemistry. 2018 Jun 12;24(33):8268-8274. doi: 10.1002/chem.201705651. Epub 2018 May 2.
In 2015, a novel way to convert photoconvertible fluorescent proteins was reported that uses the intercept of blue and far-red light instead of traditional violet or near-UV light illumination. This Minireview describes and contrasts this distinct two-step mechanism termed primed conversion with traditional photoconversion. We provide a comprehensive overview of what is known to date about primed conversion and focus on the molecular requirements for it to take place. We provide examples of its application to axially confined photoconversion in complex tissues as well as super-resolution microscopy. Further, we describe why and when it is useful, including its advantages and disadvantages, and give an insight into potential future development in the field.
2015 年，报道了一种将光转化型荧光蛋白转化的新方法，该方法使用蓝光和远红光的交点代替传统的紫光或近紫外光照射。这篇综述描述并对比了这种称为预转化的独特两步机制与传统的光转化。我们全面概述了目前已知的预转化的情况，并重点介绍了其发生所需的分子要求。我们提供了将其应用于复杂组织中的轴向限制光转化以及超分辨率显微镜的示例。此外，我们还描述了它何时何地有用，包括其优缺点，并深入探讨了该领域未来的潜在发展。
