Nakada S, Graves P N, Palese P
Virus Res. 1986 May;4(3):263-73. doi: 10.1016/0168-1702(86)90005-5.
It has previously been shown that the shortest RNA (RNA 7) of influenza C viruses codes for a nonstructural (NSI) protein (Nakada et al. (1985) J. Virol. 56, 221-226). Experiments reported here indicate that RNA 7 also directs the synthesis of a second nonstructural protein via a spliced mRNA. The amino terminal 62 codons of this NS2 protein appear to be shared with the NS1 protein and the carboxyl terminal 59 amino acids are unique (derived from a +1 open reading frame in the mRNA). Although the size of the C virus NS2 protein is comparable to that of the A and B virus NS2 proteins, the overall arrangement of the C virus NS gene is quite different from that of the A and B virus NS genes. The second (+1) open reading frame of the C virus NS gene is completely overlapped by that of the NS1 protein, whereas the second (+1) open reading frame of the A and B virus NS genes extends to the 3' end of the RNA and only partially (or in some strains not at all) overlaps the NS1 open reading frame.
先前的研究表明，丙型流感病毒最短的RNA（RNA 7）编码一种非结构（NS1）蛋白（中田等人，（1985年）《病毒学杂志》56卷，221 - 226页）。本文报道的实验表明，RNA 7还通过剪接的mRNA指导第二种非结构蛋白的合成。这种NS2蛋白的氨基末端62个密码子似乎与NS1蛋白相同，而羧基末端59个氨基酸是独特的（源自mRNA中的一个+1开放阅读框）。虽然丙型病毒NS2蛋白的大小与甲型和乙型病毒NS2蛋白相当，但丙型病毒NS基因的总体排列与甲型和乙型病毒NS基因有很大不同。丙型病毒NS基因的第二个（+1）开放阅读框与NS1蛋白的完全重叠，而甲型和乙型病毒NS基因的第二个（+1）开放阅读框延伸到RNA的3'末端，仅部分（或在某些毒株中根本不）与NS1开放阅读框重叠。
