Cheng H, Delespesse G
Am J Reprod Immunol Microbiol. 1986 May;11(1):1-5. doi: 10.1111/j.1600-0897.1986.tb00019.x.
Concanavalin A (Con A) was used to study the suppressor function of human umbilical cord blood T cells. The suppressor activity and the phenotype of Con A-activated adult and cord T cells were compared using allogeneic adult mononuclear cell (MNC) response to pokeweed mitogen (PWM), phytohemagglutinin (PHA), and alloantigen as indicator culture systems. It was found that Con A-activated adult suppressor T cells were predominantly T8+ cells and suppressed PWM-induced IgG production by adult lymphocytes, as well as PHA- and alloantigen-induced proliferation of allogeneic adult MNC. Con A-activated cord T cells, however, exhibited no significant suppression in any of the three indicator systems. Furthermore, using Con A-activated adult T8+ cells as a source of suppression, the proliferative response of cord lymphocytes to PHA and alloantigen were as susceptible to suppression as those of adult lymphocytes. These results, together with previous findings that PWM-induced cord suppressor T cells were predominantly T4+ cell-mediated suppression, suggest that fetus/newborn lymphocytes achieved their selective inhibition on adult lymphocytes through the T4+ suppressor cell circuit.
刀豆球蛋白A（Con A）被用于研究人脐带血T细胞的抑制功能。使用同种异体成人单核细胞（MNC）对商陆丝裂原（PWM）、植物血凝素（PHA）和同种异体抗原的反应作为指示培养系统，比较Con A激活的成人和脐带T细胞的抑制活性和表型。结果发现，Con A激活的成人抑制性T细胞主要是T8 + 细胞，可抑制成人淋巴细胞由PWM诱导的IgG产生，以及PHA和同种异体抗原诱导的同种异体成人MNC增殖。然而，Con A激活的脐带T细胞在这三种指示系统中均未表现出明显的抑制作用。此外，以Con A激活的成人T8 + 细胞作为抑制源，脐带淋巴细胞对PHA和同种异体抗原的增殖反应与成人淋巴细胞一样容易受到抑制。这些结果，连同之前关于PWM诱导的脐带抑制性T细胞主要是T4 + 细胞介导的抑制作用的发现，表明胎儿/新生儿淋巴细胞通过T4 + 抑制细胞回路对成人淋巴细胞实现了选择性抑制。
