Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Talca, Chile.
Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, Talca, Chile.
Food Res Int. 2018 Mar;105:10-18. doi: 10.1016/j.foodres.2017.10.074. Epub 2017 Nov 8.
The Chilean white strawberry (Fragaria chiloensis spp. chiloensis f. chiloensis) is a semi-domesticated strawberry with high polyphenol content and antioxidant activity occurring in southern Chile. The aim of this work was to compare the composition and bioactivity of the polyphenol-enriched fruit extract (PEE) before and after simulated gastrointestinal digestion (GID). Results show a decrease by >50% in the total phenolic (TP) content at the end of the GID, compared to the non-digested PEE. A reduction in the antioxidant capacity of the PEEs was observed after GID by means of DPPH, FRAP, TEAC and anion superoxide assays. After simulated GID the PEE significantly inhibited α-glucosidase with an IC value of 3.13μg/mL. The inhibition of pancreatic lipase was reduced by 95% after GID. All the PEEs did not show inhibitory effect towards α-amylase throughout the GID. In the same way, the PEEs did not significantly protect human gastric adenocarcinoma (AGS) cells against HO-induced stress. Thirty eight compounds were tentatively identified in the non-digested PEE. The compounds that were more affected by the simulated GID were simple phenolics. After the GID, only 33 and 25 compounds were detected, in the gastric and intestinal steps, respectively. These results evidence the changes elicited by GID on the bioactivity and polyphenolic composition of the white strawberry.
智利白草莓（Fragaria chiloensis spp. chiloensis f. chiloensis）是一种半驯化的草莓，具有高多酚含量和抗氧化活性，分布在智利南部。本工作旨在比较模拟胃肠道消化（GID）前后富含多酚的果实提取物（PEE）的组成和生物活性。结果表明，与未经消化的 PEE 相比，GID 结束时总酚（TP）含量下降超过 50%。通过 DPPH、FRAP、TEAC 和阴离子超氧化物测定法，观察到 GID 后 PEE 的抗氧化能力降低。经过模拟 GID 后，PEE 对α-葡萄糖苷酶的抑制作用显著，IC 值为 3.13μg/mL。GID 后，对胰腺脂肪酶的抑制作用降低了 95%。所有 PEE 在 GID 过程中对α-淀粉酶均没有抑制作用。同样，PEE 对 HO 诱导的人胃腺癌细胞（AGS）应激没有显著的保护作用。在未经消化的 PEE 中，共推测出 38 种化合物。受模拟 GID 影响较大的化合物是简单酚类。在 GID 后，仅在胃和肠步骤中分别检测到 33 和 25 种化合物。这些结果证明了 GID 对白草莓生物活性和多酚组成的变化。
