Dept. Sanitat Animal, Facultat de Veterinària, Universitat Autònoma de Barcelona, Avda dels Turons, 08193 Bellaterra, Barcelona, Spain.
Parc Taulí Hospital Universitari, Institut de Investigació i Innovació Sanitària Parc Taulí I3PT, 08208 Sabadell, Barcelona, Spain.
Ticks Tick Borne Dis. 2018 Mar;9(3):629-631. doi: 10.1016/j.ttbdis.2018.02.002. Epub 2018 Feb 8.
To determine the prevalence of exposure to Rickettsia massiliae/Bar29 and Rickettsia conorii in wild red foxes, we collected blood samples and ticks from 135 foxes shot in different game reserve areas in Catalonia. To detect SFG rickettsia in Rhipicephalus sanguineus complex ticks collected from the foxes, we used real-time polymerase chain reaction (PCR) to screen for ompA gene and a tick-borne bacteria flow chip technique based on multiplex PCR. Serum samples were positive for antibodies against spotted fever group (SFG) rickettsiae in 68 (50.3%). Molecular techniques identified R. massiliae in 107 ticks, R. aeschlimannii in 3 ticks, and R. slovaca in one tick; no R. conorii was identified in any of the ticks analyzed. We conclude that red foxes can carry ticks with SFG rickettsia.
为了确定马赛利亚立克次体/巴尔 29 株和康氏立克次体在野生红狐中的暴露流行率，我们从加泰罗尼亚不同狩猎保护区中射杀的 135 只狐狸身上采集了血液样本和蜱虫。为了在从狐狸身上采集的革蜱复合种群蜱虫中检测 SFG 立克次体，我们使用实时聚合酶链反应（PCR）筛查ompA 基因和基于多重 PCR 的蜱传细菌流芯片技术。68 份（50.3%）血清样本对斑点热群（SFG）立克次体抗体呈阳性。分子技术在 107 只蜱虫中鉴定出了马赛利亚立克次体，3 只蜱虫中鉴定出了阿氏立克次体，1 只蜱虫中鉴定出了斯洛伐克立克次体；在分析的所有蜱虫中均未鉴定出康氏立克次体。我们得出结论，红狐可以携带 SFG 立克次体的蜱虫。
