Zhang Xiao Fang, Xu Hong Mei, Han Lei, Li Nian Bing, Luo Hong Qun
Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University.
Anal Sci. 2018;34(2):149-153. doi: 10.2116/analsci.34.149.
The detection of disease-related DNA is of great significance for early and accurate diagnosis and therapy. In this work, we successfully achieved the sensitive detection of target DNA based on a thioflavin T (ThT)-induced G-quadruplex fluorescent biosensor. ThT, a water-soluble fluorescent dye, can induce G-rich sequences to form G-quadruplexes and obtain an obviously enhanced fluorescence. In this work, it was employed to construct a biosensor for the detection of HIV. When the target HIV existed, the hairpin DNA probes would be opened in succession and release the completely exposed G-rich sequence to combine with ThT. The simple and rapid biosensor performed satisfactory selectivity; it also exhibited sensitivity with a detection limit of 2.4 nM. With good performance in human serum, we believe that this optical biosensor has the potential to be applied to the practical detection of target DNA.
疾病相关DNA的检测对于早期准确诊断和治疗具有重要意义。在这项工作中，我们基于硫黄素T（ThT）诱导的G-四链体荧光生物传感器成功实现了对目标DNA的灵敏检测。ThT是一种水溶性荧光染料，可诱导富含G的序列形成G-四链体并获得明显增强的荧光。在这项工作中，它被用于构建一种检测HIV的生物传感器。当目标HIV存在时，发夹DNA探针会相继打开并释放出完全暴露的富含G的序列与ThT结合。这种简单快速的生物传感器表现出令人满意的选择性；其检测限为2.4 nM，也具有灵敏度。鉴于在人血清中具有良好性能，我们认为这种光学生物传感器有潜力应用于目标DNA的实际检测。
