Papadogiannakis N, Johnsen S A, Olding L B
Cell Immunol. 1986 Aug;101(1):51-61. doi: 10.1016/0008-8749(86)90185-1.
In a previous study we reported that cord blood lymphocytes show lower OKT3 responses as compared to their mothers and to other, unrelated adults. In the study reported here, we investigated the interactions between lymphocytes and adherent accessory cells in OKT3-stimulated cultures of newborn (cord), maternal, and other adult peripheral blood mononuclear leukocytes (PBML) and determined the following. (1) Removal of adherent cells (AC), by two cycles of plastic adherence or by nylon wool columns, impaired the OKT3-induced proliferation of maternal/adult cells, but significantly enhanced the OKT3 responsiveness of cord cells. (2) Addition of indomethacin, and other prostaglandin (PG) synthesis inhibitors, caused a more than twofold augmentation of cord PBML OKT3 responses, but had only a small, if any, enhancing effect on maternal/adult PBML. Cord PBML cultures deprived of AC were no longer enhanced by indomethacin. (3) Exogenous PGE2 (1.4 X 10(-6) through 1.4 X 10(-9) M) strongly inhibited OKT3-induced proliferation of maternal, cord, and adult PBML, at a wide range of antibody concentrations (5-100 ng/ml). However, an obvious difference in the extent of PG-mediated inhibition was observed among these three populations, and the order of PG sensitivity, from most to least sensitive, was cord greater than maternal greater than adult. (4) Purified interleukin-1 (IL-1) could not replace the accessory function of AC in the OKT3-induced proliferation of maternal/adult lymphocytes. In contrast, IL-1 increased by greater than 50% the OKT3 responsiveness of cord PBML in the absence, but not in the presence, of cord monocytes. Our observations strongly argue for a distinct, predominantly suppressive function of cord monocytes as compared to maternal/adult monocytes in OKT3-induced mitogenesis, and indicate prostaglandins as major mediators of this suppression.
在之前的一项研究中，我们报告称，与母亲及其他无关成年人相比，脐血淋巴细胞对OKT3的反应较低。在本文所报告的研究中，我们研究了新生儿（脐带血）、母亲及其他成年人外周血单个核白细胞（PBML）在OKT3刺激培养下淋巴细胞与贴壁辅助细胞之间的相互作用，并得出以下结果。（1）通过两轮塑料贴壁或尼龙毛柱去除贴壁细胞（AC），会损害母亲/成年人细胞对OKT3诱导的增殖，但显著增强脐血细胞对OKT3的反应性。（2）添加吲哚美辛及其他前列腺素（PG）合成抑制剂，可使脐带血PBML对OKT3的反应增强两倍以上，但对母亲/成年人PBML的增强作用很小（若有增强作用的话）。去除AC的脐带血PBML培养物不再受吲哚美辛增强。（3）外源性PGE2（1.4×10−6至1.4×10−9 M）在广泛的抗体浓度（5 - 100 ng/ml）下，强烈抑制母亲、脐带血和成年人PBML对OKT3诱导的增殖。然而，在这三个群体中观察到PG介导的抑制程度存在明显差异，PG敏感性顺序为脐带血＞母亲＞成年人，从最敏感到最不敏感。（4）纯化的白细胞介素-1（IL-1）不能替代AC在母亲/成年人淋巴细胞对OKT3诱导的增殖中的辅助功能。相反，在没有脐带血单核细胞但有脐带血PBML存在的情况下，IL-1可使脐带血PBML对OKT3的反应性增加超过50%。我们的观察结果有力地表明，与母亲/成年人单核细胞相比，脐带血单核细胞在OKT3诱导的有丝分裂中具有独特的、主要为抑制性的功能，并表明前列腺素是这种抑制的主要介质。
