Xu Siliang, Liu Xiaoqiang, Gao Lingling, Xu Boqun, Li Jianmin, Gao Chao, Cui Yugui, Liu Jiayin
State Key Laboratory of Reproductive Medicine, Center for Clinical Reproductive Medicine, First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Exp Ther Med. 2018 Feb;15(2):1982-1988. doi: 10.3892/etm.2017.5612. Epub 2017 Dec 11.
As a multifunctional protein involved in numerous biological processes, Set is expressed in several embryonic and adult organs. Furthermore, Set is overexpressed in numerous types of human cancers, including acute myeloid leukemia, breast cancer and pancreatic cancer. The expression of Set in germ cells is involved in gonad development, and the overexpression of Set has been observed in polycystic ovaries. In order to elucidate the physiological and pathological roles of Set, a Set transgenic mouse model was developed, in which the global overexpression of Set in adult tissues could be induced via the Cre/loxP system with the precise deletion of the Stop fragment in double-transgenic hybrids. This result was then confirmed by genotypical and protein analysis using polymerase chain reaction and bioluminescence imaging. In conclusion, the conditional Set transgenic mice carrying a reporter system were successfully generated. The transgenic mice open a new window for the further investigation of the function of Set using tissue-specific Cre mice and inducible Cre systems.
作为一种参与众多生物学过程的多功能蛋白质，Set在多个胚胎和成年器官中表达。此外，Set在多种人类癌症中过表达，包括急性髓性白血病、乳腺癌和胰腺癌。Set在生殖细胞中的表达参与性腺发育，并且在多囊卵巢中观察到Set过表达。为了阐明Set的生理和病理作用，构建了Set转基因小鼠模型，在该模型中，成年组织中Set的整体过表达可通过Cre/loxP系统诱导，双转基因杂种中的Stop片段被精确删除。然后通过聚合酶链反应和生物发光成像进行基因分型和蛋白质分析证实了这一结果。总之，成功构建了携带报告系统的条件性Set转基因小鼠。这些转基因小鼠为使用组织特异性Cre小鼠和诱导性Cre系统进一步研究Set的功能打开了一扇新窗口。
