Zhao Jun, Li Huan, Deng Hao, Zhu Li, Zhou Bingyu, Yang MeiQiong, Liu QianRu, Luo GuoQun, Yang Yunxia, Ma WenMin
Assisted Reproductive Technology Center, Foshan Maternal and Child Health Care Hospital, Foshan, China.
Oncotarget. 2017 Nov 24;9(4):5105-5110. doi: 10.18632/oncotarget.23696. eCollection 2018 Jan 12.
Varicocele-related sperm damages are usually caused by oxidative stresses. Growing evidence indicates that lncRNA growth arrested DNA-damage inducible gene 7 (gadd7) is involved in the regulation of the oxidative stress responses. In this study, we measured the expression level of gadd7 in the sperm and found that the expression of gadd7 was significantly up-regulated in patients with varicocele compared with the healthy control. The relative expression level of gadd7 was negatively correlated with the sperm count. Overexpression of gadd7 suppressed cell proliferation and promoted cell apoptosis in mouse spermatocyte-derived cell lines GC-1 and GC-2. Furthermore, the protein level of Bax was raised while Bcl2 expression was reduced after overexpression of gadd7. This work provides a potential novel insight for the varicocele-related sperm impairment and male infertility.
精索静脉曲张相关的精子损伤通常由氧化应激引起。越来越多的证据表明，长链非编码RNA生长停滞DNA损伤诱导基因7（gadd7）参与氧化应激反应的调节。在本研究中，我们检测了精子中gadd7的表达水平，发现精索静脉曲张患者中gadd7的表达与健康对照组相比显著上调。gadd7的相对表达水平与精子数量呈负相关。gadd7的过表达抑制了小鼠精母细胞来源的细胞系GC-1和GC-2中的细胞增殖并促进了细胞凋亡。此外，gadd7过表达后，Bax蛋白水平升高而Bcl2表达降低。这项工作为精索静脉曲张相关的精子损伤和男性不育提供了一个潜在的新见解。
