1College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, 450002 China.
2Medical Microbiology Laboratory, Henan Provincial People's Hospital, Zhengzhou, 450002 China.
Antimicrob Resist Infect Control. 2018 Feb 2;7:18. doi: 10.1186/s13756-018-0310-8. eCollection 2018.
The plasmid-encoded multidrug efflux pump confers bacterial resistance primarily to olaquindox, quinolones, and chloramphenicol. The aims of this study were to investigate the prevalence of among isolates from dogs, cats, and humans in Henan, China and the susceptibilities of isolates to common antibiotics.
From 2012 to 2014, a total of 600 samples which included 400 rectal samples and 200 clinical human specimens were tested for the presence of . All isolates were screened for genes by PCR and sequencing. The MICs of 11 antimicrobial agents were determined by the broth microdilution method. A total of 30 representative -positive isolates were subjected to ERIC-PCR and MLST. Additionally, conjugation experiments and southern hybridizations were performed.
Of 270 isolates, 58.5% (62/106) of the isolates from dogs, 56.25% (36/64) of the isolates from cats, and 42.0% (42/100) of the isolates from humans were positive for the . Olaquindox resistance was found for 85.7%-100% of -positive isolates. Of -positive isolates from dogs, cats, and humans, ciprofloxacin resistance was inspected for 85.8%, 59.1%, and 93.8%, respectively. Several -positive isolates were demonstrated by ERIC-PCR and MLST, and have high similarity. Phylogenetic analysis showed that -positive isolates could be divided into 7 major clusters. -positive conjugants were obtained, southern hybridization verified that the gene complex was primarily located on plasmids.
In conclusion, -positive isolates were widespread in animals and humans in Henan, China. Carriage of on plasmids of isolates may facilitate the emergence of multidrug resistant and its transmission via horizontal transfer, and might pose a potential threat to public health.
质粒编码的多药外排泵主要导致细菌对奥拉喹多、喹诺酮类和氯霉素耐药。本研究旨在调查中国河南地区犬、猫和人分离株中 的流行情况以及 分离株对常见抗生素的敏感性。
2012 年至 2014 年，共检测了 600 份样本，包括 400 份直肠样本和 200 份临床人标本，以检测 的存在。所有分离株均通过 PCR 和测序筛选 基因。采用肉汤微量稀释法测定 11 种抗菌药物的 MIC。对 30 株代表阳性分离株进行 ERIC-PCR 和 MLST。此外，还进行了接合实验和 Southern 杂交。
在 270 株分离株中，犬分离株阳性率为 58.5%（62/106），猫分离株阳性率为 56.25%（36/64），人分离株阳性率为 42.0%（42/100）。85.7%-100%的 阳性分离株对奥拉喹多耐药。犬、猫和人分离株中，对环丙沙星耐药的分别为 85.8%、59.1%和 93.8%。通过 ERIC-PCR 和 MLST 检测，发现部分 阳性分离株具有较高的相似度。系统发育分析显示， 阳性分离株可分为 7 个主要簇。获得了 阳性接合子，Southern 杂交证实 基因复合物主要位于质粒上。
总之，中国河南地区动物和人分离株中广泛存在 阳性。 分离株质粒上携带 基因可能有助于多药耐药的产生及其通过水平转移传播，并可能对公共卫生构成潜在威胁。
