Departament de Química Orgànica, Facultat de Farmàcia, Universitat de València, Vicent Andrés Estellés s/n. 46100 Burjassot, València, Spain.
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de València, Dr. Moliner 50, E-46100 Burjassot, València, Spain.
Appl Microbiol Biotechnol. 2018 Mar;102(6):2543-2561. doi: 10.1007/s00253-018-8827-6. Epub 2018 Feb 12.
Yeast display is a powerful strategy that consists in exposing peptides or proteins of interest on the cell surface of this microorganism. Ever since initial experiments with this methodology were carried out, its scope has extended and many applications have been successfully developed in different science and technology fields. Several yeast display systems have been designed, which all involve introducting into yeast cells the gene fusions that contain the coding regions of a signal peptide, an anchor protein, to properly attach the target to the cell surface, and the protein of interest to be exposed, all of which are controlled by a strong promoter. In this work, we report the description of such elements for the alternative systems introduced by focusing particularly on anchor proteins. The comparisons made between them are included whenever possible, and the main advantages and inconveniences of each one are discussed. Despite the huge number of publications on yeast surface display and the revisions published to date, this topic has not yet been widely considered. Finally, given the growing interest in developing systems for non-Saccharomyces yeasts, the main strategies reported for some are also summarized.
酵母展示是一种强大的策略，包括将感兴趣的肽或蛋白质暴露在这种微生物的细胞表面上。自从最初进行这项方法的实验以来，它的范围已经扩大，并且在不同的科学和技术领域成功开发了许多应用。已经设计了几种酵母展示系统，所有这些系统都涉及将包含信号肽、锚定蛋白编码区的基因融合物导入酵母细胞中，以便将目标物正确地附着到细胞表面，并将感兴趣的蛋白质暴露出来，所有这些都受强启动子的控制。在这项工作中，我们报告了通过特别关注锚定蛋白引入的替代系统的这些元件的描述。只要有可能，就包括了它们之间的比较，并讨论了每种方法的主要优点和缺点。尽管关于酵母表面展示的出版物数量庞大，并且迄今为止已经发表了修订版，但这个主题尚未得到广泛考虑。最后，考虑到人们对开发非酿酒酵母系统的兴趣日益浓厚，也总结了一些报道的主要策略。
