Spezia Adachi Lauren N, Vercelino Rafael, de Oliveira Carla, Scarabelot Vanessa L, de Souza Andressa, Medeiros Liciane F, Cioato Stefania G, Caumo Wolnei, Torres Iraci L S
Graduate Program in Medicine, Medical Sciences, School of Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Laboratory of Pain Pharmacology and Neuromodulation: Preclinical Researchs, Department of Pharmacology, Institute of Basic Health Sciences, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil; Animal Experimentation Unit, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
Laboratory of Pain Pharmacology and Neuromodulation: Preclinical Researchs, Department of Pharmacology, Institute of Basic Health Sciences, Universidade Federal Do Rio Grande Do Sul, Porto Alegre, RS, Brazil; Centro Universitário FADERGS, Health and Wellness School Laureate International Universities, Porto Alegre, RS, Brazil.
J Acupunct Meridian Stud. 2018 Jun;11(3):97-106. doi: 10.1016/j.jams.2018.01.004. Epub 2018 Feb 6.
The present study aimed to determine whether isoflurane interferes with the analgesic effects of acupuncture (Ac) and electroacupuncture (EA), using a neuropathic pain (NP) rat model. In total, 140 male Wistar rats were used; isoflurane-induced nociceptive response was evaluated using the von Frey test, serum calcium-binding protein β (S100β) levels and nerve growth factor (NGF) levels in the left sciatic nerve. The NP model was induced by chronic constriction injury of the sciatic nerve at 14 days after surgery. Treatment was initiated after NP induction with or without isoflurane anesthesia (20 min/day/8 days). The von Frey test was performed at baseline, 14 days postoperatively, and immediately, 24 h, and 48 h after the last treatment. Results of the nociceptive test and three-way analysis of variance were analyzed by generalized estimating equations, the Bonferroni test, followed by Student-Newman-Keuls or Fisher's least significant difference tests for comparing biochemical parameters (significance defined as p ≤ 0.05). At baseline, no difference was noted in the nociceptive response threshold among all groups. Fourteen days after surgery, compared with other groups, NP groups showed a decreased pain threshold, confirming establishment of NP. Ac and EA enhanced the mechanical pain threshold immediately after the last session in the NP groups, without anesthesia. Isoflurane administration caused increased nociceptive threshold in all groups, and this effect persisted for 48 h after the last treatment. There was an interaction between the independent variables: pain, treatments, and anesthesia in serum S100β levels and NGF levels in the left sciatic nerve. Isoflurane enhanced the analgesic effects of Ac and EA and altered serum S100β and left sciatic nerve NGF levels in rats with NP.
本研究旨在使用神经性疼痛（NP）大鼠模型，确定异氟烷是否会干扰针刺（Ac）和电针（EA）的镇痛效果。总共使用了140只雄性Wistar大鼠；通过von Frey试验、血清钙结合蛋白β（S100β）水平和左坐骨神经中的神经生长因子（NGF）水平评估异氟烷诱导的伤害性反应。在手术后14天，通过坐骨神经慢性压迫损伤诱导NP模型。在NP诱导后，在有或无异氟烷麻醉（每天20分钟/共8天）的情况下开始治疗。在基线、术后14天以及最后一次治疗后立即、24小时和48小时进行von Frey试验。通过广义估计方程、Bonferroni检验对伤害性试验结果和三因素方差分析进行分析，随后进行Student-Newman-Keuls检验或Fisher最小显著差异检验以比较生化参数（显著性定义为p≤0.05）。在基线时，所有组之间的伤害性反应阈值没有差异。手术后14天，与其他组相比，NP组的疼痛阈值降低，证实NP模型建立。在无麻醉的情况下，Ac和EA在NP组最后一次治疗后立即提高了机械性疼痛阈值。异氟烷给药使所有组的伤害性阈值升高，并且这种作用在最后一次治疗后持续48小时。在血清S100β水平和左坐骨神经NGF水平方面，自变量（疼痛、治疗和麻醉）之间存在相互作用。异氟烷增强了Ac和EA的镇痛效果，并改变了NP大鼠的血清S100β和左坐骨神经NGF水平。
