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Fluorescence In Situ Hybridization of Cryosectioned Oocytes.冷冻切片卵母细胞的荧光原位杂交
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Whole-Mount In Situ Hybridization of Oocytes.卵母细胞的全胚胎原位杂交
Cold Spring Harb Protoc. 2018 Mar 1;2018(3):2018/3/pdb.prot097014. doi: 10.1101/pdb.prot097014.
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Three-dimensional ultrastructural analysis of RNA distribution within germinal granules of Xenopus.非洲爪蟾生发颗粒内RNA分布的三维超微结构分析
Dev Biol. 2002 Jan 1;241(1):79-93. doi: 10.1006/dbio.2001.0488.
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A transient asymmetric distribution of XNOA 36 mRNA and the associated spectrin network bisects Xenopus laevis stage I oocytes along the future A/V axis.XNOA36mRNA 的短暂非对称分布和相关的血影蛋白网络将非洲爪蟾 I 期卵母细胞沿未来的 A/V 轴二等分。
Eur J Cell Biol. 2010 Jul;89(7):525-36. doi: 10.1016/j.ejcb.2009.12.007. Epub 2010 Mar 12.
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Using in vivo imaging to measure RNA mobility in Xenopus laevis oocytes.利用体内成像技术测量非洲爪蟾卵母细胞中的RNA移动性。
Methods. 2016 Apr 1;98:60-65. doi: 10.1016/j.ymeth.2015.11.003. Epub 2015 Nov 6.
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Whole-Mount Immunofluorescence for Visualizing Endogenous Protein and Injected RNA in Oocytes.用于可视化卵母细胞中内源性蛋白质和注射RNA的全组织免疫荧光法。
Cold Spring Harb Protoc. 2018 Oct 1;2018(10):pdb.prot097022. doi: 10.1101/pdb.prot097022.
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Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes.青蛙和昆虫卵母细胞中细胞骨架及与细胞骨架结合的RNA可视化
Methods Mol Biol. 2016;1457:179-90. doi: 10.1007/978-1-4939-3795-0_13.
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Chromosomal storage of the RNA-editing enzyme ADAR1 in Xenopus oocytes.非洲爪蟾卵母细胞中RNA编辑酶ADAR1的染色体储存
Mol Biol Cell. 2005 Jul;16(7):3377-86. doi: 10.1091/mbc.e05-01-0016. Epub 2005 Apr 20.
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A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes.
Methods Mol Biol. 2016;1405:99-107. doi: 10.1007/978-1-4939-3393-8_10.
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GLUT Characterization Using Frog Xenopus laevis Oocytes.利用非洲爪蟾卵母细胞对葡萄糖转运蛋白（GLUT）进行特性分析
Methods Mol Biol. 2018;1713:45-55. doi: 10.1007/978-1-4939-7507-5_4.
引用本文的文献
1
L-bodies are RNA-protein condensates driving RNA localization in oocytes.L 体是 RNA-蛋白质凝聚物，可驱动卵母细胞中的 RNA 定位。
Mol Biol Cell. 2021 Dec 1;32(22):ar37. doi: 10.1091/mbc.E21-03-0146-T. Epub 2021 Oct 6.
2
Cryosectioning and Immunostaining of Embryonic Tissues.胚胎组织的冷冻切片和免疫染色。
Cold Spring Harb Protoc. 2021 Sep 1;2021(9):pdb.prot107151. doi: 10.1101/pdb.prot107151.
3
Using the Oocyte Toolbox.使用卵母细胞工具箱。
Cold Spring Harb Protoc. 2020 Apr 1;2020(4):095844. doi: 10.1101/pdb.top095844.
本文引用的文献
1
Isolation of Oocytes.卵母细胞的分离
Cold Spring Harb Protoc. 2018 Feb 1;2018(2):pdb.prot095851. doi: 10.1101/pdb.prot095851.
2
Two-color fluorescence in situ hybridization using chromogenic substrates in .在……中使用显色底物的双色荧光原位杂交
Biotechniques. 2016 Nov 1;61(5):263-268. doi: 10.2144/000114475. eCollection 2016.
3
Directional transport is mediated by a Dynein-dependent step in an RNA localization pathway.定向运输是由 RNA 定位途径中的 Dynein 依赖性步骤介导的。
PLoS Biol. 2013;11(4):e1001551. doi: 10.1371/journal.pbio.1001551. Epub 2013 Apr 30.
4
Electron microscopy, immunostaining, cytoskeleton visualization, in situ hybridization, and three-dimensional reconstruction of Xenopus oocytes.电子显微镜、免疫染色、细胞骨架可视化、原位杂交和非洲爪蟾卵母细胞的三维重建。
Methods. 2010 May;51(1):11-9. doi: 10.1016/j.ymeth.2009.12.003. Epub 2009 Dec 16.
5
Imaging individual mRNA molecules using multiple singly labeled probes.使用多个单标记探针成像单个mRNA分子。
Nat Methods. 2008 Oct;5(10):877-9. doi: 10.1038/nmeth.1253. Epub 2008 Sep 21.
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Multiple kinesin motors coordinate cytoplasmic RNA transport on a subpopulation of microtubules in Xenopus oocytes.多个驱动蛋白马达协同作用，在非洲爪蟾卵母细胞微管的一个亚群上协调细胞质RNA转运。
Dev Cell. 2008 Sep;15(3):426-436. doi: 10.1016/j.devcel.2008.06.014. Epub 2008 Sep 4.
7
The Xenopus ELAV protein ElrB represses Vg1 mRNA translation during oogenesis.非洲爪蟾的ELAV蛋白ElrB在卵子发生过程中抑制Vg1 mRNA的翻译。
Mol Cell Biol. 2005 Oct;25(20):9028-39. doi: 10.1128/MCB.25.20.9028-9039.2005.
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Potential structural role of non-coding and coding RNAs in the organization of the cytoskeleton at the vegetal cortex of Xenopus oocytes.非编码RNA和编码RNA在非洲爪蟾卵母细胞植物皮质细胞骨架组织中的潜在结构作用。
Development. 2005 Aug;132(15):3445-57. doi: 10.1242/dev.01919. Epub 2005 Jul 6.
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Xenopus Staufen is a component of a ribonucleoprotein complex containing Vg1 RNA and kinesin.非洲爪蟾Staufen是一种核糖核蛋白复合体的组成成分，该复合体包含Vg1 RNA和驱动蛋白。
Development. 2004 Jul;131(13):3035-45. doi: 10.1242/dev.01170. Epub 2004 May 26.
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Assembly of the nuclear transcription and processing machinery: Cajal bodies (coiled bodies) and transcriptosomes.核转录与加工机制的组装：卡哈尔体（卷曲小体）与转录体。
Mol Biol Cell. 1999 Dec;10(12):4385-402. doi: 10.1091/mbc.10.12.4385.
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