Yano Rintaro, Inadomi Chiaki, Luo Lan, Goto Shinji, Hara Tetsuya, Li Tao-Sheng
Department of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan.
Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Japan.
PLoS One. 2018 Feb 13;13(2):e0192733. doi: 10.1371/journal.pone.0192733. eCollection 2018.
For general anesthesia, pre-oxygenation is routinely performed prior to intubation. It is well-known that ischemic/hypoxic preconditioning induces stem cell mobilization and protects against ischemia/reperfusion (I/R) injury. In this study, we investigated the effect of transient oxygenation on stem cell mobilization and I/R injury of the heart. Mice were exposed to 100% oxygen for 5 or 20 minutes. We evaluated the number of c-kit+ stem/progenitor cells and the levels of SDF-1α and VEGF in peripheral blood at 1, 3, 6, and 24 hours after oxygenation. We also induced I/R injury of the heart at 3 hours post-oxygenation for 5 minutes and then examined stem cell recruitment and fibrotic changes in the heart 3 or 14 days later. The number of c-kit+ cells in peripheral blood was significantly increased at 1 or 24 hours after oxygenation for either 5 or 20 minutes. Oxygenation for 5 or 20 minutes did not significantly change the SDF-1α level measured in plasma. However, the plasma VEGF level was decreased at 3 hours post-oxygenation for 20 minutes (p = 0.051). Oxygenation for 5 minutes did not significantly alter the fibrotic area or cell apoptosis. Although oxygenation for 5 minutes increased the number of c-kit+ cells in hearts damaged by I/R injury, this difference was not significant between groups due to large variation between individuals (p = 0.14). Although transient oxygenation induces stem cell mobilization, it does not appear to protect against I/R injury of the heart in mice.
对于全身麻醉，插管前常规进行预充氧。众所周知，缺血/缺氧预处理可诱导干细胞动员并预防缺血/再灌注（I/R）损伤。在本研究中，我们调查了短暂充氧对干细胞动员及心脏I/R损伤的影响。将小鼠暴露于100%氧气中5或20分钟。我们评估了充氧后1、3、6和24小时外周血中c-kit+干细胞/祖细胞的数量以及SDF-1α和VEGF的水平。我们还在充氧后3小时诱导心脏I/R损伤5分钟，然后在3或14天后检查心脏中的干细胞募集和纤维化变化。充氧5或20分钟后，外周血中c-kit+细胞的数量在1或24小时显著增加。充氧5或20分钟并未显著改变血浆中测得的SDF-1α水平。然而，充氧20分钟后3小时血浆VEGF水平降低（p = 0.051）。充氧5分钟并未显著改变纤维化面积或细胞凋亡。尽管充氧5分钟增加了I/R损伤所致心脏中c-kit+细胞的数量，但由于个体间差异较大，两组间差异不显著（p = 0.14）。尽管短暂充氧可诱导干细胞动员，但它似乎并不能预防小鼠心脏的I/R损伤。
