Bosch Dolors, Rodríguez Marcela A, Depalo Laura, Avilla Jesús
Sustainable Plant Protection Program (IRTA), Lleida, Spain.
Departamento de Zoología, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Castilla, Concepción, Chile.
J Econ Entomol. 2018 Apr 2;111(2):844-852. doi: 10.1093/jee/toy020.
The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the key pest on pome fruit and walnut orchards worldwide. Its resistance to available insecticides has been widely reported. Chlorantraniliprole is an anthranilic diamide that was introduced in European countries in 2008-2009 and acts by activating the insect's ryanodine receptors. The aims of this study were to determine the baseline susceptibility of European populations of C. pomonella to chlorantraniliprole, to establish the discriminant concentrations (DC) to check the possible development of resistance, and to know the role of cytochrome P450 monooxygenases (P450) in the possible susceptibility decrease of field populations to the insecticide. Ten field populations from Spain along with others were used to calculate the baseline response of larvae to chlorantraniliprole incorporated into the diet. A pooled probit line was calculated, and three DC were established: 0.3 mg a.i./kg (close to the LC50), 1.0 mg a.i./kg (close to the LC90), and 10 mg a.i./kg diets (threefold the LC99). The DC were used to test the susceptibility of 27 field populations from France, Germany, Hungary, Italy, and Spain. The corrected mortality observed in all cases ranged within the expected interval, even with Spanish populations that showed between 12.1 and 100.0% of individuals with high P450 activity. However, the mortality caused by the DC0.3 decreased as the mean P450 activity increased. Field populations resistant to other insecticides were susceptible to chlorantraniliprole. The determined baseline codling moth susceptibility is a valuable reference for tracking possible future alterations in the efficacy of the insecticide.
苹果蠹蛾，Cydia pomonella (L.)（鳞翅目：卷蛾科），是全球梨果和核桃果园的主要害虫。其对现有杀虫剂的抗性已被广泛报道。氯虫苯甲酰胺是一种邻氨基苯甲酰胺类杀虫剂，于2008 - 2009年在欧洲国家引入，通过激活昆虫的兰尼碱受体起作用。本研究的目的是确定欧洲苹果蠹蛾种群对氯虫苯甲酰胺的基线敏感性，建立判别浓度（DC）以检查抗性的可能发展，并了解细胞色素P450单加氧酶（P450）在田间种群对该杀虫剂敏感性可能降低中的作用。使用来自西班牙的10个田间种群以及其他种群来计算幼虫对掺入饲料中的氯虫苯甲酰胺的基线反应。计算了一条合并的概率线，并建立了三个判别浓度：0.3毫克有效成分/千克（接近LC50）、1.0毫克有效成分/千克（接近LC90）和10毫克有效成分/千克饲料（LC99的三倍）。这些判别浓度用于测试来自法国、德国、匈牙利、意大利和西班牙的27个田间种群的敏感性。在所有情况下观察到的校正死亡率都在预期范围内，即使是西班牙种群中P450活性高的个体比例在12.1%至100.0%之间的情况也是如此。然而随平均P450活性增加，DC0.3引起的死亡率降低。对其他杀虫剂有抗性的田间种群对氯虫苯甲酰胺敏感。确定的苹果蠹蛾基线敏感性是追踪该杀虫剂未来可能的药效变化的有价值参考。
