Ahlersová E, Ahlers I, Toropila M
Physiol Bohemoslov. 1986;35(3):233-41.
Adult male Wistar rats adapted to a 12:12 h light:dark regimen, fed or after a 24- or 48-h fast, were decapitated at 3-h intervals during a single day. They were deprived of food at day-time intervals ensuring that on decapitation they had fasted for the same length of time, i.e. 24 or 48 h. Thyroid hormones, insulin and glucose concentrations were determined in their serum. Fasting did not significantly affect circadian thyroxine, triiodothyronine and reverse triiodothyronine rhythms compared with the findings in fed animals; 24, but not 48 hours' fasting led to a shift in the acrophase of circadian insulin and glucose oscillations compared with fed rats. The maintenance of original circadian thyroid hormones and insulin rhythm in rats which fasted for short lengths of time testifies to a dependence of the stimulus on the time of day.
适应12:12小时光照:黑暗周期的成年雄性Wistar大鼠，喂食后或禁食24或48小时，在一天内每隔3小时断头处死。在白天的不同时间段剥夺它们的食物，以确保断头时它们禁食的时间相同，即24或48小时。测定其血清中的甲状腺激素、胰岛素和葡萄糖浓度。与喂食动物的结果相比，禁食对昼夜甲状腺素、三碘甲状腺原氨酸和反三碘甲状腺原氨酸节律没有显著影响；与喂食大鼠相比，禁食24小时而非48小时导致昼夜胰岛素和葡萄糖振荡的峰相位发生偏移。短时间禁食的大鼠中昼夜甲状腺激素和胰岛素节律的维持证明了刺激对一天中时间的依赖性。
