Cui Y M, Han X H, Lin Y Y, Lv W W, Wang Y L
Pharmazie. 2017 Jun 1;72(6):329-333. doi: 10.1691/ph.2017.7450.
It has been reported that calcium hydroxide can induce proliferation, migration, and mineralization in dental pulp stem cells (DPSCs), but the underlying molecular mechanisms are still unclear. In this study, we sought to explore the role of calcium hydroxide in the cell proliferation and directional differentiation of DPSCs and to study the regulatory effect of NF-κB, p38MAPK, and Wnt signaling on differentiation of DPSCs. CCK8 cell assay, Wound Healing Assay, and Alkaline Phosphatase Staining Assay were respectively used to determine the proliferation rate, migration and ALP expression of DPSCs. Alizarin Red Staining Assay was used to observe the mineralization of DPSCs. RT-PCR analysis and Western Blot Analysis displayed the expression of related fators at mRNA and protein level, respectively. In the present study, we found that NF-κB, p38MAPK, and Wnt signaling could abolish calcium hydroxide-induced proliferation of DPSCs. The inhibition of NF-κB, p38MAPK, and Wnt signaling suppressed the migration, ALP expression, and mineralization of DPSCs. NF-κB, p38MAPK, and Wnt signaling involved in directional differentiation of DPSCs. Moverover, calcium hydroxide could activate NF-κB, p38MAPK, and Wnt pathway by regulating TNF-α. Our study showed that NF-κB, p38MAPK, and Wnt signaling pathway were involved in calcium hydroxide-induced proliferation, migration, mineralization, and osteogenic differentiation in DPSCs. Calcium hydroxide affected NF-κB, p38MAPK, and Wnt pathway by regulating TNF-α.
据报道，氢氧化钙可诱导牙髓干细胞（DPSCs）增殖、迁移和矿化，但其潜在分子机制仍不清楚。在本研究中，我们试图探讨氢氧化钙在DPSCs细胞增殖和定向分化中的作用，并研究NF-κB、p38MAPK和Wnt信号对DPSCs分化的调节作用。分别采用CCK8细胞检测法、伤口愈合检测法和碱性磷酸酶染色检测法测定DPSCs的增殖率、迁移能力和碱性磷酸酶（ALP）表达。茜素红染色检测法用于观察DPSCs的矿化情况。RT-PCR分析和蛋白质免疫印迹分析分别显示相关因子在mRNA和蛋白质水平的表达。在本研究中，我们发现NF-κB、p38MAPK和Wnt信号可消除氢氧化钙诱导的DPSCs增殖。抑制NF-κB、p38MAPK和Wnt信号可抑制DPSCs的迁移、ALP表达和矿化。NF-κB、p38MAPK和Wnt信号参与DPSCs的定向分化。此外，氢氧化钙可通过调节肿瘤坏死因子-α（TNF-α）激活NF-κB、p38MAPK和Wnt通路。我们的研究表明，NF-κB、p38MAPK和Wnt信号通路参与氢氧化钙诱导的DPSCs增殖、迁移、矿化和成骨分化。氢氧化钙通过调节TNF-α影响NF-κB、p38MAPK和Wnt通路。
