Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
J Nat Med. 2018 Mar;72(2):563-569. doi: 10.1007/s11418-018-1175-0. Epub 2018 Feb 13.
In a previous study, we found that both synthetic 3-O-methylquercetin (3MQ) and 3,4',7-O-trimethylquercetin (34'7TMQ) increased extracellular melanin content. 34'7TMQ increased the activity of melanogenic enzymes by stimulating the p38 pathway and the expression of microphthalmia-associated transcription factor (MITF). In contrast, 3MQ increased the activity of melanogenic enzymes without the involvement of MITF, which suggests that 3MQ inhibits the degradation of melanogenic enzymes. In the present study, we investigated the effects of 3MQ and 34'7TMQ on melanogenesis in normal human melanocytes and using a commercial three-dimensional (3D) skin model system. Both 3MQ and 34'7TMQ elongated the dendrites of normal human melanocytes from a Caucasian donor, but did not stimulate melanogenesis in the melanocytes. In the 3D skin model, which included melanocytes from an Asian donor, 3MQ and 34'7TMQ increased and elongated the melanocytes and showed a tendency to stimulate melanogenesis. These results suggest that 3MQ and 34'7TMQ could be put to practical use in skin care products and agents aimed at preventing hair graying.
在之前的一项研究中，我们发现合成的 3-O-甲基槲皮素（3MQ）和 3,4',7-O-三甲基槲皮素（34'7TMQ）都能增加细胞外黑色素含量。34'7TMQ 通过刺激 p38 通路和小眼畸形相关转录因子（MITF）的表达来增加黑素生成酶的活性。相比之下，3MQ 通过不涉及 MITF 来增加黑素生成酶的活性，这表明 3MQ 抑制了黑素生成酶的降解。在本研究中，我们研究了 3MQ 和 34'7TMQ 对正常人类黑素细胞的黑素生成的影响，并使用商业三维（3D）皮肤模型系统。3MQ 和 34'7TMQ 均使来自白种人供体的正常人黑素细胞的树突伸长，但并未刺激黑素细胞中的黑素生成。在包括来自亚洲供体的黑素细胞的 3D 皮肤模型中，3MQ 和 34'7TMQ 增加并伸长了黑素细胞，并表现出刺激黑素生成的趋势。这些结果表明，3MQ 和 34'7TMQ 可实际用于旨在预防白发的皮肤护理产品和制剂中。
