Van Borm Steven, Steensels Mieke, Mathijs Elisabeth, Yinda Claude Kwe, Matthijnssens Jelle, Lambrecht Bénédicte
Directorate Viral Diseases, Veterinary and Agrochemical Research Center CODA-CERVA, Brussels, Belgium.
Laboratory of Viral Metagenomics, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium.
Arch Virol. 2018 Jun;163(6):1701-1703. doi: 10.1007/s00705-018-3761-6. Epub 2018 Feb 13.
Using random high-throughput RNA sequencing, the complete coding sequence of a novel picorna-like virus (a 9,228-nt contig containing 212,202 reads) was determined from a blackbird (Turdus merula) infected with Usutu virus. This sequence shares only 36% amino acid sequence identity with its closest homolog, arivirus 1, (an unclassified member of the order Picornavirales), and shares its dicistronic genome arrangement. The new virus was therefore tentatively named "blackbird arilivirus" (ari-like virus). The nearly complete genome sequence consists of at least 9,228 nt and contains two open reading frames (ORFs) encoding the nonstructural polyprotein (2235 amino acids) and structural polyprotein (769 amino acids). Two TaqMan RT-qPCR assays specific for ORF1 confirmed the presence of high levels of this novel virus in the original sample. Nucleotide composition analysis suggests that blackbird arilivirus is of dietary (plant) origin.
通过随机高通量RNA测序，从感染乌苏图病毒的黑鹂（欧亚鸲）中确定了一种新型微小核糖核酸病毒样病毒的完整编码序列（一个包含212,202条读数的9228个核苷酸的重叠群）。该序列与其最接近的同源物阿里病毒1（微小核糖核酸病毒目未分类成员）仅具有36%的氨基酸序列同一性，并具有双顺反子基因组排列。因此，这种新病毒被暂命名为“黑鹂阿里病毒”（阿里样病毒）。近乎完整的基因组序列至少由9228个核苷酸组成，包含两个开放阅读框（ORF），分别编码非结构多聚蛋白（2235个氨基酸）和结构多聚蛋白（769个氨基酸）。两种针对ORF1的TaqMan RT-qPCR检测方法证实原始样本中存在高水平的这种新型病毒。核苷酸组成分析表明黑鹂阿里病毒起源于食物（植物）。
