Faculty of Life Sciences, Kyoto Sangyo University, Kyoto, Japan.
Adv Exp Med Biol. 2018;1046:339-351. doi: 10.1007/978-981-10-7311-3_17.
Pioneered by the classical mouse embryonic stem cells (ESCs), various stem cell lines representing the peri- and postimplantation stages of embryogenesis have been established. To gain insight into the gene regulatory network operating in these cells, we first investigated epiblast stem cells (EpiSCs), performing ChIP-seq analysis for five major transcription factors (TFs) involved in epiblast regulation. The analysis indicated that SOX2-POU5F1 TF pairs highlighted in mouse ESCs are not the major players in other stem cells. The major acting transcription factors shift from SOX2/POU5F1 in mouse ESCs to ZIC2/OTX2 in EpiSCs, and this shift is primed in ESCs by binding of ZIC2 at relevant genomic positions that later function as enhancers.
由经典的小鼠胚胎干细胞 (ESC) 开创，已经建立了各种代表胚胎发生的植入前和植入后阶段的干细胞系。为了深入了解这些细胞中运行的基因调控网络，我们首先研究了滋养外胚层干细胞 (EpiSCs)，对涉及滋养外胚层调节的五个主要转录因子 (TF) 进行了 ChIP-seq 分析。该分析表明，在小鼠 ESC 中突出显示的 SOX2-POU5F1 TF 对不是其他干细胞中的主要参与者。主要作用的转录因子从小鼠 ESC 中的 SOX2/POU5F1 转变为 EpiSCs 中的 ZIC2/OTX2，这种转变由 ZIC2 在相关基因组位置的结合引发，这些位置后来作为增强子发挥作用。
