Gao Jian, Wang Ai-Rong, Jiang Xiao-Ping, Zhang Jiu-Xun, Zhang Ye-Wang
College of Petroleum and Chemical Engineering, Qinzhou University, Qinzhou 535011, People's Republic of China.
School of Pharmacy.
J Nanosci Nanotechnol. 2018 Jul 1;18(7):4852-4857. doi: 10.1166/jnn.2018.15266.
Immobilization of glycerol dehydrogenase (GDH) from Serratia marcescens H30 onto epoxy functional magnetic nanoparticles by covalent attachment was carried out. The optimal immobilization conditions were obtained as follows: enzyme/support 6.08 mg/g, temperature 25 °C, pH 7.0 and time 8 h. Under these conditions, a high immobilization yield above 90% was obtained. The characterization of the immobilized GDH indicated that enhanced pH and thermal stability were achieved. Kinetic parameters Km of free and immobilized GDH were determined as 10.35 mM and 15.76 mM, respectively. The immobilized GDH retained about 85% initial activity after ten cycles. These results suggested that GDH immobilized onto magnetic nanoparticles is a simple and efficient way for preparation of stable enzyme. And the immobilized GDH has potential applications in the production of DHA.
通过共价连接将粘质沙雷氏菌H30的甘油脱氢酶（GDH）固定在环氧官能化磁性纳米颗粒上。获得了如下最佳固定化条件：酶/载体为6.08 mg/g、温度25℃、pH 7.0以及时间8小时。在这些条件下，获得了高于90%的高固定化产率。固定化GDH的表征表明其pH稳定性和热稳定性得到了增强。游离和固定化GDH的动力学参数Km分别测定为10.35 mM和15.76 mM。固定化GDH在十个循环后保留了约85%的初始活性。这些结果表明，固定在磁性纳米颗粒上的GDH是制备稳定酶的一种简单有效的方法。并且固定化GDH在DHA生产中具有潜在应用。
