Kanimozhi K, Basha S Khaleel, Kumari V Sugantha, Kaviyarasu K
Department of Chemistry, Auxilium College (Autonomous), Vellore 632006, India.
Department of Chemistry, C. Abdul Hakeem College, Melvisharam 632509, India.
J Nanosci Nanotechnol. 2018 Jul 1;18(7):4916-4922. doi: 10.1166/jnn.2018.15306.
Freeze drying and salt leaching methods were applied to fabricate Chitosan/Poly(vinyl alcohol)/Carboxymethyl cellulose (CPCMC) biomimetic porous scaffolds for soft tissue engineering. The properties of these scaffolds were investigated and compared to those by freeze drying and salt leaching methods respectively. The salt-leached CS/PVA/CMC scaffolds were easily formed into desired shapes with a uniformly distributed and interconnected pore structure with an average pore size. The mechanical strength of the scaffolds increased with the porosity, and were easily modulated by the addition of carboxymethyl cellulose. The morphology of the porous scaffolds observed using a SEM exhibited good porosity and interconnectivity of pores. MTT assay using L929 fibroblast cells demonstrated that the cell viability of the porous scaffold was good. Scaffolds prepared by salt leached method show larger swelling capacity, and mechanical strength, potent antibacterial activity and more cell viability than freeze dried method. It is found that salt leaching method has distinguished characteristics of simple, efficient, feasible and less economic than freeze dried scaffolds.
采用冷冻干燥法和盐析法制备用于软组织工程的壳聚糖/聚乙烯醇/羧甲基纤维素（CPCMC）仿生多孔支架。分别研究了这些支架的性能，并与冷冻干燥法和盐析法制备的支架性能进行比较。盐析法制备的CS/PVA/CMC支架易于成型为所需形状，具有均匀分布且相互连通的孔结构以及平均孔径。支架的机械强度随孔隙率增加而提高，并且通过添加羧甲基纤维素易于调节。使用扫描电子显微镜观察的多孔支架形态显示出良好的孔隙率和孔连通性。使用L929成纤维细胞进行的MTT试验表明多孔支架的细胞活力良好。与冷冻干燥法相比，盐析法制备的支架具有更大的溶胀能力、机械强度、强效抗菌活性和更高的细胞活力。发现盐析法具有简单、高效、可行且比冷冻干燥支架成本更低的显著特点。
