Wu Pian, Huang Ruixue, Li Guiyin, He Yayuan, Chen Cuimei, Xiao Wen, Ding Ping
Xiang Ya School of Public Health, Central South University, Changsha, Hunan, 410078, China.
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi, 541014, China.
J Nanosci Nanotechnol. 2018 May 1;18(5):3654-3659. doi: 10.1166/jnn.2018.14673.
This study prepared an innovative 3-mercaptopropionic acid modified ZnSe/ZnS core/shell quantum dots (MPA-ZnSe/ZnS QDs), and established a rapid fluorescence method to detect the E. coli cells count by using MPA-ZnSe/ZnS QDs as fluorescence probe. The formulation variables and process were optimized using the response surface methodology (RSM). Fluorescence microscopy was used to obtain fluorescence microscope images of MPA-ZnSe/ZnS QDs that bind to bacteria. The fluorescence peak intensity increases with increasing cells count in the range of 101-108 CFU/mL. Compared with the traditional based on fluorescent detection methods, this method is more convenient and useful in the bacterial count determination.
本研究制备了一种创新的3-巯基丙酸修饰的ZnSe/ZnS核壳量子点（MPA-ZnSe/ZnS QDs），并建立了一种以MPA-ZnSe/ZnS QDs作为荧光探针检测大肠杆菌细胞数量的快速荧光方法。采用响应面法（RSM）对配方变量和工艺进行了优化。利用荧光显微镜获得了与细菌结合的MPA-ZnSe/ZnS QDs的荧光显微镜图像。在101-108 CFU/mL范围内，荧光峰强度随细胞数量增加而增加。与传统的基于荧光的检测方法相比，该方法在细菌计数测定中更方便、实用。
