Rosberg S, Norjavaara E, Sender Baum M, Khan I
Acta Endocrinol (Copenh). 1986 Aug;112(4):565-70. doi: 10.1530/acta.0.1120565.
Adenylate cyclase activity was studied in membranes from isolated corpora lutea of defined ages obtained from pregnant mare's serum gonadotropin treated rats and the effects of luteinizing hormone (LH), isoproterenol, guanylylimidodiphosphate (Gpp (NH)p), fluoride and forskolin were compared. The effect of LH on adenylate cyclase activity increased with the luteal age up to nine days of age, while the effect of isoproterenol increased dramatically during the first days, reaching a maximum at 2-3 days of age and then declined. Forskolin potentiated the effects of both LH and isoproterenol without affecting the patterns of age-dependency. The effect of forskolin itself was fairly constant during the luteal phase, indicating a relatively constant amount of the catalytic unit in the corpus luteum. The effects of fluoride and Gpp(NH)p on the other hand increased markedly during the first days and then remained constant for the rest of the period studied. These results suggest that the regulatory Ns-protein develops during the first days of luteal life. It is speculated that the close correlation between the development of beta-adrenergic response and the development of Ns-protein are causally related.
研究了从孕马血清促性腺激素处理的大鼠分离得到的特定年龄黄体膜中的腺苷酸环化酶活性，并比较了促黄体生成素（LH）、异丙肾上腺素、鸟苷酰亚胺二磷酸（Gpp(NH)p）、氟化物和福斯高林的作用。LH对腺苷酸环化酶活性的作用随黄体年龄增加至9日龄而增强，而异丙肾上腺素的作用在最初几天急剧增加，在2 - 3日龄达到最大值，然后下降。福斯高林增强了LH和异丙肾上腺素的作用，而不影响年龄依赖性模式。福斯高林本身的作用在黄体期相当恒定，表明黄体中催化单位的量相对恒定。另一方面，氟化物和Gpp(NH)p的作用在最初几天显著增加，然后在研究的其余时期保持恒定。这些结果表明，调节性Ns蛋白在黄体生命的最初几天发育。据推测，β-肾上腺素能反应的发育与Ns蛋白的发育之间的密切相关性存在因果关系。
