de Camp Nora Vanessa, Hense Florian, Lecher Bernd, Scheu Helmut, Bergeler Jürgen
Medical Center of the Johannes Gutenberg, University Mainz, Mainz, Germany.
Free University Berlin, Berlin, Germany.
Transl Neurosci. 2017 Dec 29;8:211-224. doi: 10.1515/tnsci-2017-0029. eCollection 2017.
The objective of this study was to evaluate the piglet and the mouse as model systems for preterm cortical development. According to the clinical context, we used non invasive EEG recordings. As a prerequisite, we developed miniaturized Ag/AgCl electrodes for full band EEG recordings in mice and verified that Urethane had no effect on EEG band power. Since mice are born with a "preterm" brain, we evaluated three age groups: P0/P1, P3/P4 and P13/P14. Our aim was to identify EEG patterns in the somatosensory cortex which are distinguishable between developmental stages and represent a physiologic brain development. In mice, we were able to find clear differences between age groups with a simple power analysis of EEG bands and also for phase locking and power spectral density. Interhemispheric coherence between corresponding regions can only be seen in two week old mice. The canolty maps for piglets as well as for mice show a clear PAC (phase amplitude coupling) pattern during development. From our data it can be concluded that analytic tools relying on network activity, as for example PAC (phase amplitude coupling) are best suited to extract basic EEG patterns of cortical development across species.
本研究的目的是评估仔猪和小鼠作为早产皮层发育的模型系统。根据临床背景，我们使用了无创脑电图记录。作为前提条件，我们开发了用于小鼠全频段脑电图记录的小型化银/氯化银电极，并证实乌拉坦对脑电图频段功率没有影响。由于小鼠出生时大脑处于“早产”状态，我们评估了三个年龄组：P0/P1、P3/P4和P13/P14。我们的目的是识别体感皮层中的脑电图模式，这些模式在发育阶段之间是可区分的，并代表生理性脑发育。在小鼠中，通过对脑电图频段进行简单的功率分析以及锁相和功率谱密度分析，我们能够发现不同年龄组之间的明显差异。相应区域之间的半球间相干性仅在两周龄的小鼠中可见。仔猪和小鼠的脑地形图在发育过程中均显示出明显的相位-幅度耦合（PAC）模式。从我们的数据可以得出结论，依赖于网络活动的分析工具，例如相位-幅度耦合（PAC），最适合提取跨物种皮层发育的基本脑电图模式。
