Department of Mechanical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4L7, Canada.
Biomater Sci. 2018 Feb 27;6(3):683-694. doi: 10.1039/c7bm01050h.
A magnet array is employed to manipulate diamagnetic cells that are contained in paramagnetic medium to demonstrate for the first time the contactless bioprinting of three-dimensional (3D) cellular structures and co-cultures of breast cancer MCF-7 and endothelial HUVEC at prescribed locations on tissue culture treated well plates. Sequential seeding of different cell lines and the spatial displacement of the magnet array creates co-cultured cellular structures within a well without using physically intrusive well inserts. Both monotypic and co-culture experiments produce morphologically rich 3D cell structures that are otherwise absent in regular monolayer cell cultures. The magnetic contactless bioprinting of cells provides further insight into cell behaviour, invasion strategies and transformations that are useful for potential applications in drug screening, 3D cell culture formation and tissue engineering.
采用磁体阵列来操控包含在顺磁介质中的抗磁性细胞，首次在组织培养处理过的平板孔中以预定位置展示了非接触式生物打印三维（3D）细胞结构和乳腺癌 MCF-7 和内皮细胞 HUVEC 的共培养物。通过不同细胞系的顺序接种和磁体阵列的空间位移，在不使用物理侵入式孔内插物的情况下，在孔内创建共培养的细胞结构。无论是单型还是共培养实验，都会产生形态丰富的 3D 细胞结构，而这些在常规单层细胞培养中是不存在的。细胞的磁性非接触式生物打印为细胞行为、入侵策略和转化提供了进一步的了解，这对于药物筛选、3D 细胞培养形成和组织工程等潜在应用非常有用。
