a College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
b Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, 450002, China.
Genome. 2018 May;61(5):337-347. doi: 10.1139/gen-2017-0114. Epub 2018 Feb 15.
Chicken muscle quality is one of the most important factors determining the economic value of poultry, and muscle development and growth are affected by genetics, environment, and nutrition. However, little is known about the molecular regulatory mechanisms of long non-coding RNAs (lncRNAs) in chicken skeletal muscle development. Our study aimed to better understand muscle development in chickens and thereby improve meat quality. In this study, Ribo-Zero RNA-Seq was used to investigate differences in the expression profiles of muscle development related genes and associated pathways between Gushi (GS) and Arbor Acres (AA) chickens. We identified two muscle tissue specific expression lncRNAs. In addition, the target genes of these lncRNAs were significantly enriched in certain biological processes and molecular functions, as demonstrated by Gene Ontology (GO) analysis, and these target genes participate in five signaling pathway, as revealed by an analysis of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Taken together, these data suggest that different lncRNAs might be involved in regulating chicken muscle development and growth and provide new insight into the molecular mechanisms of lncRNAs.
鸡肉品质是决定家禽经济价值的最重要因素之一，肌肉的发育和生长受到遗传、环境和营养的影响。然而，关于长链非编码 RNA（lncRNA）在鸡骨骼肌发育中的分子调控机制知之甚少。本研究旨在更好地了解鸡的肌肉发育，从而改善肉质。本研究使用 Ribo-Zero RNA-Seq 技术研究了固始鸡（GS）和爱拔益加（AA）鸡肌肉发育相关基因及其相关通路的表达谱差异。我们鉴定了两个肌肉组织特异性表达的 lncRNA。此外，通过基因本体论（GO）分析，这些 lncRNA 的靶基因在某些生物学过程和分子功能中显著富集，这些靶基因参与五个信号通路，如京都基因与基因组百科全书（KEGG）数据库分析所示。综上所述，这些数据表明，不同的 lncRNA 可能参与调节鸡的肌肉发育和生长，并为 lncRNA 的分子机制提供新的见解。
