Department of Plastic Surgery, 1st Affiliated Hospital of Bengbu Medical College, 287 Changhuai Road, Bengbu, Anhui 233000, PR China.
Department of Anatomy, Bengbu Medical College, Bengbu, Anhui 233000, PR China.
Int J Biol Macromol. 2018 Jun;112:1014-1020. doi: 10.1016/j.ijbiomac.2018.02.077. Epub 2018 Feb 13.
Congenital muscular torticollis (CMT) is a neck deformity that involves shortening of sternocleidomastoid muscle (SCM) characterized by muscle atrophy and interstitial fibrosis. To investigate wheatear Botulinum toxin type A (BTA) has anti-fibrotic effects in CMT, we established acquired muscular torticollis that mimetics CMT in rabbit by intra-SCM injection of anhydrous alcohol. The treatment groups received BTA (2.5units or 5units) injection into the fibrotic SCM. The shortening and thickening of SCM were recorded by B-mode ultrasound. Changes in Col1A1, Fn, α-SMA expression were determined by immunohistochemistry. In vitro studies, TGF-β induced NIH3T3 fibroblasts were used to evaluate anti-fibrosis effect of BTA. Expression of the myofibroblast marker α-SMA and fibrosis markers Col1A1 and Fn were detected by Western blotting and quantitative RT-PCR. Our results showed that BTA injection attenuated shortening and thickening of fibrotic SCM. Elevated expression of Col1A1, Fn, α-SMA were confirmed in this fibrotic muscle model but reversed after BTA injection. Similar results observed in TGF-β induced NIH3T3 fibroblasts in both mRNA and protein levels. In conclusion, our results suggested that BTA could be a promising agent against SCM fibrosis in CMT through regulating fibroblast and inhibiting myofibroblast differentiation.
先天性肌性斜颈（CMT）是一种颈部畸形，涉及胸锁乳突肌（SCM）缩短，其特征为肌肉萎缩和间质纤维化。为了研究肉毒毒素 A 型（BTA）是否对 CMT 具有抗纤维化作用，我们通过向兔 SCM 内注射无水乙醇建立了类似于 CMT 的获得性肌性斜颈。治疗组将 BTA（2.5 单位或 5 单位）注入纤维化的 SCM。通过 B 型超声记录 SCM 的缩短和增厚。通过免疫组织化学检测 Col1A1、Fn、α-SMA 表达的变化。在体外研究中，使用 TGF-β 诱导的 NIH3T3 成纤维细胞评估 BTA 的抗纤维化作用。通过 Western blot 和定量 RT-PCR 检测成纤维细胞标记物α-SMA 和纤维化标记物 Col1A1 和 Fn 的表达。我们的结果表明，BTA 注射可减轻纤维化 SCM 的缩短和增厚。在这种纤维化肌肉模型中证实了 Col1A1、Fn、α-SMA 的表达升高，但 BTA 注射后得到逆转。在 TGF-β 诱导的 NIH3T3 成纤维细胞中，在 mRNA 和蛋白水平上均观察到类似的结果。总之，我们的结果表明，BTA 可能通过调节成纤维细胞和抑制肌成纤维细胞分化成为 CMT 中 SCM 纤维化的一种有前途的治疗药物。
