College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5;196:141-147. doi: 10.1016/j.saa.2018.01.067. Epub 2018 Feb 5.
A novel method based on amino acid ionic liquids (AAILs) as an additive synchronous fluorescence spectrometry is proposed for simultaneous determination of magnolol (MN) and honokiol (HN) in traditional Chinese medicine Houpu. The overlapping fluorescence spectrum of MN and HN could be completely separated in the AAILs medium. Experiment parameters (the type and concentration of AAILs, pH values and temperature) were discussed. The detection limits of MN and HN reached 1.46ng/mL, 0.92ng/mL and the recovery rates ranged from 98.6%-100.7%, 99.7%-100.6%, respectively. This methods was successfully employed for simultaneously determination of MN and HN in real samples. No significant differences could be found in the results of this method and the pharmacopoeia of People's Republic of China 2015 (Ch.P.2015). The experiment mechanisms were discussed by the Gaussian simulation and fluorescence quantum yield.
提出了一种基于氨基酸离子液体（AAILs）作为添加剂同步荧光光谱法，用于同时测定中药厚朴中厚朴酚（MN）和和厚朴酚（HN）。在 AAILs 介质中，MN 和 HN 的重叠荧光光谱可以完全分离。实验参数（AAILs 的类型和浓度、pH 值和温度）进行了讨论。MN 和 HN 的检测限分别达到 1.46ng/mL 和 0.92ng/mL，回收率范围分别为 98.6%-100.7%和 99.7%-100.6%。该方法成功地用于实际样品中 MN 和 HN 的同时测定。与 2015 年版《中华人民共和国药典》（Ch.P.2015）相比，该方法的结果无显著性差异。通过高斯模拟和荧光量子产率讨论了实验机制。
