Murali Adhigan, Haridharan Neelamegan, Babu Pombala Suresh, Jayaveni Sivalingam, Jaisankar Sellamuthu N
Polymer Division, Council of Scientific and Industrial Research (CSIR)-Central Leather Research Institute (CLRI), Adyar, Chennai 600020, Tamil Nadu, India.
Department of Biotechnology, Council of Scientific and Industrial Research (CSIR)-Central Leather Research Institute (CLRI), Adyar, Chennai 600020, Tamil Nadu, India.
J Nanosci Nanotechnol. 2018 Feb 1;18(2):959-966. doi: 10.1166/jnn.2018.13959.
We report rhodamine based fluorophore derivative for the stable dispersion of single-walled carbon nanotubes (SWCNTs), which can afford better fluorescent label to carbon nanotubes (CNTs). The nanotubes-fluorophore conjugates are helpful in achieving stable dispersion in polar and non-polar solvents with intense fluorescence. The product was characterized through NMR, Mass spectrometry, Raman, XPS, SEM, AFM and Fluorescence measurements. The formation of SWCNT-g- Rhodamine was confirmed by the presence of D and G bands in Raman spectrum. The alkyl and aryl groups in the range of 14.8, 17.6, 38.1 and 96.3 ppm confirms the grafting of the nanocomposite through NMR. The morphological studies were carried out intensively for analyzing SWCNTs stable dispersion and the results from EDAX measurements shows the elements weight% of C: 35.09 and N: 30.1 concludes that SWCNTs are completely grafted onto rhodamine derivatives. The application of SWCNTs fluorophore conjugates were analyzed by cell viability studies using MTT assay and exhibits less toxic compare to other functionalized CNTs. The viability of percentage increases with decrease in the concentration of SWCNT-COCl with 91.7% of live cells even after 24 h at a concentration of 250 μg for SWCNT-g-Rhodamine. The fluorescent images obtained during viability analysis shows enhanced fluorescence for living cells in case of SWCNT-g-Rhodamine compared to SWCNT-COCl, which clearly shows the utility of decorating nanotubes with fluorophore. This research work further extends its application for molecular sensing and other biological process.
我们报道了一种基于罗丹明的荧光团衍生物，用于单壁碳纳米管（SWCNTs）的稳定分散，它能为碳纳米管（CNTs）提供更好的荧光标记。纳米管-荧光团共轭物有助于在极性和非极性溶剂中实现稳定分散，并具有强烈的荧光。通过核磁共振（NMR）、质谱、拉曼光谱、X射线光电子能谱（XPS）、扫描电子显微镜（SEM）、原子力显微镜（AFM）和荧光测量对产物进行了表征。拉曼光谱中D带和G带的出现证实了SWCNT-g-罗丹明的形成。核磁共振显示，在14.8、17.6、38.1和96.3 ppm范围内的烷基和芳基基团证实了纳米复合材料的接枝。对SWCNTs的稳定分散进行了深入的形态学研究，能量散射X射线光谱（EDAX）测量结果表明，碳元素重量百分比为35.09，氮元素重量百分比为30.1，这表明SWCNTs已完全接枝到罗丹明衍生物上。通过MTT法进行细胞活力研究，分析了SWCNTs荧光团共轭物的应用，结果表明与其他功能化碳纳米管相比，其毒性较小。随着SWCNT-COCl浓度的降低，活细胞百分比增加，即使在浓度为250 μg的SWCNT-g-罗丹明作用24 h后，仍有91.7%的活细胞。活力分析过程中获得的荧光图像显示，与SWCNT-COCl相比，SWCNT-g-罗丹明处理的活细胞荧光增强，这清楚地表明了用荧光团修饰纳米管的实用性。这项研究工作进一步扩展了其在分子传感和其他生物过程中的应用。
