Argraves W S, Pytela R, Suzuki S, Millán J L, Pierschbacher M D, Ruoslahti E
J Biol Chem. 1986 Oct 5;261(28):12922-4.
The fibronectin receptor is a complex of two cell surface glycopeptides that mediate the binding of cells to fibronectin substrata. To study the structure of this receptor, we have isolated cDNA clones coding for the human fibronectin receptor alpha subunit from a lambda gt11 placental cDNA library. The cDNAs code for 229 amino acids from the COOH terminus of the alpha subunit. The deduced sequence has a hydrophobic region with properties characteristic of a membrane-spanning domain. From the membrane-spanning domain to the COOH terminus are 23-28 amino acids that are likely to constitute the cytoplasmic domain. These results establish the fibronectin receptor alpha subunit as an integral membrane protein.
纤连蛋白受体是由两种细胞表面糖肽组成的复合物，介导细胞与纤连蛋白底物的结合。为了研究该受体的结构，我们从λgt11胎盘cDNA文库中分离出编码人纤连蛋白受体α亚基的cDNA克隆。这些cDNA编码α亚基COOH末端的229个氨基酸。推导的序列有一个具有跨膜结构域特征的疏水区。从跨膜结构域到COOH末端有23 - 28个氨基酸，可能构成细胞质结构域。这些结果确定纤连蛋白受体α亚基为一种整合膜蛋白。
