Ishizaka S T, Carnaud C, Stutman O
J Immunol. 1986 Oct 1;137(7):2093-9.
The ability of various (C57BL/6J X CBA/HT6T6)F1 spleen cell subpopulations to induce tolerance to allogeneic histocompatibility antigens after injection into neonatal CBA/HT6T6 mice was examined. The requirements for tolerization of cytotoxic T lymphocyte precursors (CTLp) and IL 2-producing helper T cell precursors (IL 2Tp) appear to be coordinated but not identical. CTLp frequencies measured in limiting dilution analysis (LDA) were found to be decreased by 90 to 99% in mice injected neonatally with unseparated or a variety of semiallogeneic spleen cell fractions, including T cells, T cell-depleted spleen, the Ig+ and Ig- fractions of nylon-adherent, T-depleted spleen cells, Sephadex-G10 (G10)-nonadherent spleen cells, and T-depleted allogeneic C57BL/6J spleen cells. In contrast, IL 2Tp showed tolerization only after neonatal injection of unseparated or T cell-depleted F1 spleen cells, and not after injection of T or B cells or of G10-nonadherent or T-depleted allogeneic spleen cells. These studies show that the CTLp and IL 2Tp compartments have different requirements for neonatal tolerization, which appear to correlate with the presence of cells expressing class I or class II alloantigens in the inoculum: all spleen cell types tested were capable of tolerizing the CTLp compartment, whereas only whole spleen and T-depleted spleen cells could tolerize IL 2Tp; donor T cells, although capable of inducing CTLp tolerance, are not necessary for either CTLp or IL 2Tp tolerance induction; Ig+ B cells alone are marginally effective in tolerization of IL 2Tp, and G10-nonadherent cells are ineffective, suggesting that macrophages or another type of G10-adherent accessory cell may be required for tolerization of IL 2Tp, although it is not clear whether they are sufficient; and tolerization of CTLp can occur in the presence of a normal IL 2Tp compartment when certain inocula, such as T cells, are used for tolerance induction at birth.
研究了将各种（C57BL/6J×CBA/HT6T6）F1脾细胞亚群注射到新生CBA/HT6T6小鼠体内后，诱导对同种异体组织相容性抗原产生耐受的能力。细胞毒性T淋巴细胞前体（CTLp）和产生白细胞介素2（IL-2）的辅助性T细胞前体（IL-2Tp）产生耐受的条件似乎相互协调但并不相同。在有限稀释分析（LDA）中测得，新生期注射未分离的或各种半同种异体脾细胞组分（包括T细胞、T细胞耗尽的脾细胞、尼龙黏附的T细胞耗尽脾细胞的Ig+和Ig-组分、Sephadex-G10（G10）非黏附脾细胞以及T细胞耗尽的同种异体C57BL/6J脾细胞）的小鼠中，CTLp频率降低了90%至99%。相比之下，IL-2Tp仅在新生期注射未分离的或T细胞耗尽的F1脾细胞后才出现耐受，而在注射T细胞或B细胞、G10非黏附脾细胞或T细胞耗尽的同种异体脾细胞后则未出现耐受。这些研究表明，CTLp和IL-2Tp区室对新生期耐受有不同要求，这似乎与接种物中表达I类或II类同种异体抗原的细胞的存在有关：所有测试的脾细胞类型都能够使CTLp区室产生耐受，而只有全脾和T细胞耗尽的脾细胞能够使IL-2Tp产生耐受；供体T细胞虽然能够诱导CTLp耐受，但对于CTLp或IL-2Tp耐受诱导并非必需；单独的Ig+B细胞在使IL-2Tp产生耐受方面效果微弱，G10非黏附细胞无效，这表明巨噬细胞或另一种类型的G10黏附辅助细胞可能是使IL-2Tp产生耐受所必需的，尽管尚不清楚它们是否足够；当在出生时使用某些接种物（如T细胞）进行耐受诱导时，在正常IL-2Tp区室存在的情况下，CTLp也能产生耐受。
