c-Jun氨基末端激酶-3对纯化的线粒体电压依赖性阴离子通道的磷酸化作用改变了通道的电压依赖性。

Phosphorylation of purified mitochondrial Voltage-Dependent Anion Channel by c-Jun N-terminal Kinase-3 modifies channel voltage-dependence.

作者信息

Gupta Rajeev, Ghosh Subhendu

机构信息

Department of Physiology, All India Institute of Medical Sciences, New Delhi, India.

Department of Biophysics, University of Delhi South Campus, India.

出版信息

Biochim Open. 2017 Mar 10;4:78-87. doi: 10.1016/j.biopen.2017.03.002. eCollection 2017 Jun.

Voltage-Dependent Anion Channel (VDAC) phosphorylated by c-Jun N-terminal Kinase-3 (JNK3) was incorporated into the bilayer lipid membrane. Single-channel electrophysiological properties of the native and the phosphorylated VDAC were compared. The open probability versus voltage curve of the native VDAC displayed symmetry around the voltage axis, whereas that of the phosphorylated VDAC showed asymmetry. This result indicates that phosphorylation by JNK3 modifies voltage-dependence of VDAC.