Yang Shuang, Zheng Mingzhu, Cao Yong, Dong Yanjiao, Yaqoob Sanabil, Liu Jingsheng
Jilin Agricultural University, College of Food Science and Engineering, Changchun, China; Jilin Agricultural University, National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China.
Jilin Agricultural University, College of Food Science and Engineering, Changchun, China.
Braz J Microbiol. 2018 Jul-Sep;49(3):621-631. doi: 10.1016/j.bjm.2017.12.005. Epub 2018 Feb 13.
Cordyceps militaris 202 is a potential fungus for biotransformation zein, due to its various proteases, high tolerance and viability in nature. In this article, single factor experiment and response surface methodology were applied to optimize the liquid fermentation conditions and improve the ability of biotransformation zein. The optimized fermentation conditions were as follows: inoculum concentration of 19%, volume of liquor of 130mL/500mL and pH of 4.7. Under this condition, the degree of hydrolysis (DH) was 27.31%. The zein hydrolysates from fungi fermentation maintained a high thermal stability. Compared to the original zein, the zein hydrolysates were found to have high solubility, which most likely results in improved foaming and emulsifying properties. Overall, this research demonstrates that hydrolysis of zein by C. militaris 202 is a potential method for improving the functional properties of zein, and the zein hydrolysates can be used as functional ingredients with an increased antioxidant effect in both food and non-food applications.
蛹虫草202是一种具有生物转化玉米醇溶蛋白潜力的真菌，因为它具有多种蛋白酶，在自然界中具有高耐受性和活力。本文采用单因素实验和响应面法优化液体发酵条件，提高生物转化玉米醇溶蛋白的能力。优化后的发酵条件如下：接种浓度19%，装液量130mL/500mL，pH值4.7。在此条件下，水解度（DH）为27.31%。真菌发酵得到的玉米醇溶蛋白水解物具有较高的热稳定性。与原始玉米醇溶蛋白相比，发现玉米醇溶蛋白水解物具有高溶解性，这很可能导致其起泡和乳化性能得到改善。总体而言，本研究表明，蛹虫草202水解玉米醇溶蛋白是改善玉米醇溶蛋白功能特性的一种潜在方法，玉米醇溶蛋白水解物可作为功能性成分，在食品和非食品应用中具有增强的抗氧化作用。
