Marmolejo-León Perla, Azorín-Vega Erika Patricia, Jiménez-Mancilla Nallely, Mendoza-Nava Héctor Javier, Mitsoura Eleni, Pineda Benjamín, Torres-García Eugenio
Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de Mexico 52750, Mexico; Facultad de Medicina, Universidad Autónoma del Estado de Mexico, Toluca, Estado de Mexico 50120, Mexico.
Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de Mexico 52750, Mexico.
Appl Radiat Isot. 2018 Nov;141:156-161. doi: 10.1016/j.apradiso.2018.01.011. Epub 2018 Jan 11.
Glioblastoma contains self-renewing, tumorigenic cancer stem-like cells that contribute to tumor initiation and therapeutic resistance. The aim of this research was to estimate and compare the effectiveness ratio (α/β) of stem-like cells and differentiated glioma cells derived from the U87MG glioblastoma cell line. Cell survival experiments were obtained in a dose range of 0-20 Gy (13.52 ± 0.09 Gy/h) as a hyperfractionationated accelerated radiotherapy scheme. Biochemical characterization of the post-irradiated cells was performed by flow cytometry analysis and the percentage of stem-like cells that resisted irradiation was determined by the CD133 expression. Results showed that U87MG stem-like cells are highly proliferative and more radioresistant than the U87MG adherent group (with a lesser stem-like character), this in association with the calculated α/β ratio of 17 and 14.1, respectively.
胶质母细胞瘤含有自我更新、具有致瘤性的癌症干细胞样细胞，这些细胞有助于肿瘤的起始和治疗抗性。本研究的目的是评估和比较源自U87MG胶质母细胞瘤细胞系的干细胞样细胞和分化的胶质瘤细胞的有效率（α/β）。作为超分割加速放疗方案，在0-20 Gy（13.52±0.09 Gy/h）的剂量范围内进行细胞存活实验。通过流式细胞术分析对辐照后细胞进行生化表征，并通过CD133表达确定抵抗辐照的干细胞样细胞的百分比。结果表明，U87MG干细胞样细胞高度增殖且比U87MG贴壁组（具有较小的干细胞样特征）更具放射抗性，这分别与计算出的α/β比率17和14.1相关。
