Fluit A C, Baas P D, Jansz H S
Virology. 1986 Oct 30;154(2):357-68. doi: 10.1016/0042-6822(86)90461-7.
The nucleotide sequence requirements for termination and reinitiation of rolling circle DNA replication within the 30-bp phi X174 origin region were studied. Plasmids were constructed which contained a complete and a partial phi X174 origin region in the same orientation. The partial origin consisted of the first 16, 24, 25, 26, 27, or 28 bp of the origin region. Plasmids harboring a complete origin region are subject to rolling circle DNA replication and packaging of single-stranded plasmid DNA into phage coats in phi X174 or G4 phage infected cells. The plasmids with a complete and partial origin region were tested in these in vivo transduction systems. The results lead to the following conclusions: The phi X174 and G4 in vivo transduction systems are useful in studying termination and reinitiation of rolling circle DNA replication. The first 24 bp of the origin region are sufficient for termination of a round of rolling circle DNA replication coupled to DNA packaging. The first 16 bp, however, are not recognized as a termination signal. Reinitiation of rolling circle DNA replication coupled to DNA packaging on a partial origin region occurs with low frequency.
研究了30bp的φX174起始区域内滚环DNA复制终止和重新起始的核苷酸序列要求。构建了一些质粒，这些质粒在同一方向上包含完整和部分φX174起始区域。部分起始区域由起始区域的前16、24、25、26、27或28bp组成。携带完整起始区域的质粒在φX174或G4噬菌体感染的细胞中会经历滚环DNA复制，并将单链质粒DNA包装到噬菌体外壳中。在这些体内转导系统中测试了具有完整和部分起始区域的质粒。结果得出以下结论：φX174和G4体内转导系统可用于研究滚环DNA复制的终止和重新起始。起始区域的前24bp足以终止一轮与DNA包装偶联的滚环DNA复制。然而，前16bp不被识别为终止信号。在部分起始区域上与DNA包装偶联的滚环DNA复制重新起始发生频率较低。
