Cai Xianlei, Yao Ling, Sheng Qiyue, Jiang Luyao, Dahlgren Randy A, Wang Ting
Key Laboratory of Watershed Science and Health of Zhejiang Province, Wenzhou Medical University, Wenzhou, 325035, China.
Southern Zhejiang Water Research Institute, Wenzhou, 325035, China.
AMB Express. 2018 Feb 16;8(1):22. doi: 10.1186/s13568-018-0545-z.
Bacterial communities of biofilms growing on artificial substrates were examined at two time periods (7 and 14 days) and two locations (lentic and lotic areas) in a hypereutrophic urban river of eastern China. Previous studies in this river network indicated that variations of microbial communities were the major factor affecting the distribution of antibiotic resistant genes highlighting the importance of understanding controls of microbial communities. Bacterial communities associated with biofilms were determined using epifluorescence microscopy and high-throughput sequencing. Results showed that sampling time and site had significant effects on the abundances of surface-associated bacteria. No significant differences were found in the number of surface-associated bacteria between two substrate types (filament vs. slide). Sequencing revealed microbial communities attached to artificial substrates in a hypereutrophic urban river were composed of 80,375 OTUs, and distributed in 47 phyla. Proteobacteria and Cyanobacteria/Chloroplast were the two dominant phyla, followed by Planctomycetes, Actinobacteria, Verrucomicrobia, Firmicutes and Bacteroidetes. Taxonomic composition showed ammonia-oxidizing microorganisms, fecal indicator bacteria and pathogens enriched in attached microbial communities, especially the ammonia-oxidizing Nitrosomonas bacteria. These results indicated that there were significant temporal and intra-river heterogeneity of attached microbial community structure, but no significant difference in community composition was detected between the two substrate types.
在中国东部一条富营养化城市河流中，在两个时间段（7天和14天）和两个地点（静水和流水区域）对生长在人工基质上的生物膜细菌群落进行了研究。此前在该河网的研究表明，微生物群落的变化是影响抗生素抗性基因分布的主要因素，这凸显了了解微生物群落控制因素的重要性。使用落射荧光显微镜和高通量测序确定与生物膜相关的细菌群落。结果表明，采样时间和地点对表面相关细菌的丰度有显著影响。两种基质类型（丝状与玻片）之间表面相关细菌的数量没有显著差异。测序显示，富营养化城市河流中附着在人工基质上的微生物群落由80375个操作分类单元组成，分布在47个门中。变形菌门和蓝细菌/叶绿体是两个优势门，其次是浮霉菌门、放线菌门、疣微菌门、厚壁菌门和拟杆菌门。分类组成显示氨氧化微生物、粪便指示菌和病原体在附着的微生物群落中富集，尤其是氨氧化亚硝化单胞菌。这些结果表明，附着微生物群落结构存在显著的时间和河流内部异质性，但两种基质类型之间未检测到群落组成的显著差异。
